Objective To analysis the effect of heat shock protein 60 (HSP60) on reaction of the severity of acute coronary syndrome (ACS) at the time of admission, and to investigate the relationship between it and other risk factors with coronary heart disease (CHD). Methods 89 cases of hospitalized patients were collected from department of Cardiology, Wuxi People's Hospital affiliated to Nanjing Medical University from November 2009 to May 2010, which were divided into ACS group, stable angina pectoris (SAP) group and non-CHD group. All patients underwent coronary angiography. Patient’s information was collected in questionnaire, which contained all the clinical data of patients. Immediately HSP60 levels in human serum was measured in all patients with ELASA at the time of admission. Results There was significant difference in serum HSP60 levels in the three groups (F=7.870，p =0.001), further analysis showed HSP60 in human serum was 931.32 ± 219.23 pg / ml in ACS group, Which was significantly higher than SAP group 824.74 ± 112.62 pg / ml andnon-CHD group 743.20 ± 146.33 pg / ml (p <0.05). Correlations between HSP60 and other index which could react the severity of CHD showed, there were positive correlation between HSP60 and brain natriuretic peptide (BNP), creatine kinase MB, aspartate aminotransferase, lactate dehydrogenase, Gensini points（p <0.05）, and negative correlation with left ventricular ejection fraction（LVEF）(p <0.05). And we found that human HSP60 in serum were significantly increased in patients who were current smokers than who was never (p <0.05). Conclusions Detection of human heat shock protein 60 in ACS has important value, it can be used as a biochemical index in reaction the severity of ACS.