[Abstract] Objectine :To discuss the myocardial enzymes activity effect of the neonatal hyerbilirubinemia . Methods:60 cases with neonatal hyperbilirubinemia as a treatment group ,40 cases of the same period replacement ot the normal newborn as a control group .To monitor the two groups before and after enzyme AST CK CK-MB LDH CTNT ECG values.and to compare and analyze .Results :The higher the concentration of bilirubin enzyme activity was also high.and with the decline in total bilirubin level.the myocardial creatine phosphate and creatine kimase-MB was also gradually declining .before and after treatment enzymogram value of the treatment group were compared and there were significant difference (P<0.01). bilirubin level decline, myocardial enzymes also decline,between of normal group no difference(P>0.05).Conclusion: the neonatal hyerbilirubinemia cause rise of myocardial enzymes ,but myocardial cell uncertain irreversible damage. Pediatricians judge harm of neonatal hyperbilirubinemia to cardiac muscle except ECG neonatal jaundice in children should be as routine examination enzymogram projects to avoid children with neonatal jaundice and death due to myocardial damage.