Objective The study the effects of Clostridium ButyricumCGMCC0313.1 on the incidence and early nutrition condition of preterm infants feeding intolerance. Methods 54 premature infant in our hospital from January 2012 to December 2012 were randomly divided into study group(n＝26) and control group(n＝28).On the basis of positive treatment, the study group was given the Clostridium ButyricumCGMCC0313.1 meanwhile. To compare the incidence and nutrition index of preterm infants feeding intolerance of each group. Results The incidence of preterm infants feeding intolerance in study group was lower in study group than that in control group (19.2vs46.4)(P<0.05).There were significant difference in the time to increase weight, to regain birth weight, to start feeding、the time that daily calorie intakes reached 80Kcal/kg、the time to reach full enteral nutrition、persistence time of jaundice and the hospitalized days in study and control groups (P＜0.05). Conclusion ButyricumCGMCC0313.1 can decrease the incidence of preterm infants feeding intolerance, improve the nutrition condition of preterm infants. it was secure and effective, so is deserved to be introduced in basic hospital.