objective Wip1/p38MAPK/p53 signal pathway inbalance play an important role on the progress of tumor formation. Here we detect the mRNA and protein expression level of wide-type p53-induceed phosphatase 1, p53 and p38MAPK in non-small cell lung cancer and tumor-adjacent tissues, aim to investigate the clinical significance of wip1 expression in non-small cell lung cancer. Method Quantitative real-time RT-PCR was introduced to detect the wip1,p53 and p38MAPK mRNA expression level in 60cases’ non-small cell lung cancer and corresponding tumor-adjacent tissues, and Retrospective analysis of the relationship between wip1 mRNA expression and clinicopathological characters, western blot analysis was employed to detect the protein level of wip1, p53 and p38MAPK. Result p38MAPK and p53 mRNA expression have no difference in both tumor and tumor-adjacent group. compare with the adjacent group, wip1 mRNA were significantly higher in the tumor group(t=9.94, P<0.001) ;wip1 protein level were 0.38±0.11,1.09±0.32 in adjacent and tumor group respectively, protein level in tumor group significantly higher in tumor group(t=15.8, P<0.01). Yet p38MAPK and p53 protein expression significantly higher in the adjacent but not the tumor group(p38MAPK:t=11.1, P<0.01; p53:t=17.80, P<0.01). Wip1 mRNA overexpression related with tumor pathology and stage (p P<0.05). Conclusion wip1 was highly expressed in the early stage of non-small cell lung cancer and act as a negative regulator of p53 and p38MAPK, our investigation suggest wip1 play an important role in the formation and development of non-small cell lung cancer.