【Abstract】Objective To explore the relationship of the serum levels of Erythropoietin and wound healing of deep partial thickness burn. Methods 52 patients with moderate burns were chosen, 90 wounds of deep second degree burn were randomly selected and 30 healthy volunteers as control group.The levels of erythropoietin(EPO) were determined in control group and patients in day 2 after burns with enzyme-linked immunosorbent assay (ELISA). 52 patients were separated into 3 groups: <0.5MOM,≥0.5-≤2.5MOM and >2.5MOM according to the median multiples of EPO (MOM). wound healing rate , wound healing time and scar score 6 month after burns were evaluated. Results.The levels of EPO in patients with moderate burns was significantly higher than the healthy volunteers（P<0.05）, 9 cases were at <0.5MOM group, 33 cases were at ≥0.5-2.5≤MOM group，11 cases were at >2.5MOM group, There was significant difference at the wound healing time in the <0.5MOM group and>2.5MOM group（P<0.05）， there were significant difference at the wound healing rate and scar score in the three groups（all were P<0.05）. The correlation analysis showed the serum level of EPO was positively related with the wound healing rate（P<0.05）. the serum level of EPO was negatively correlated with the wound healing time and the scar score（all were P<0.05）. Conclusions Serum erythropoietin can promote wound healing of deep second degree burn.