Abstract Objective: To explore the relationship between plasma glycine concentration and the risk to myocardial infarction in patients with stable angina pectoris. Methods: From 2008 to 2010 collected from 2948 cases of patients with stable angina pectoris, the level of low density lipoprotein cholesterol（LDL-c），high density lipoprotein cholesterol（HDL-c），apolipoprotein B（ApoB）and apolipoprotein AI（ApoAI）in serum were tested. And the level of plasma glycine were tested by high-performance liquid chromatography-tandem mass spectrometry. And recorded each patient related risk factors (gender, age, smoking, obesity, high blood pressure, diabetes). Cox regression was used to estimate the association between plasma glycine and AMI. Results: Follow-up 5 years, there're a total of 389 cases had acute myocardial infarction. Baseline statistics found that there were lesser patients with obesity , hypertension and diabetes mellitus in patients with the higher levels of plasma glycine. Adjust risk factors related to coronary heart disease (CHD), levels of plasma glycine is inversely associated with the risk of AMI (hazard ratio per SD: 0.89; 95% CI, 0.82–0.98; P=0.017). The inverse association was generally stronger in those with apolipoprotein B, low-density lipoprotein cholesterol, or apolipoprotein AI, above the median. Conclusion: The plasma levels of glycine as an independent factor is inversely associated with the incidence of acute myocardial infarction in patients with stable angina.