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果糖-1,6-二磷酸酶在胃癌组织中的表达及其对预后的预测价值
作者:郭飞波  杨勇 
单位:湖北省天门市第一人民医院 检验科, 湖北 天门 431700
关键词:胃癌 果糖-1 6-二磷酸酶 糖酵解 瓦博格效应 预后 
分类号:R735.2
出版年·卷·期(页码):2017·36·第三期(334-339)
摘要:

目的:探讨胃癌组织果糖-1,6-二磷酸酶(FBP1)表达及其对生存预后的预测价值。方法:收集81例行手术治疗的胃癌患者胃癌组织和癌旁组织,另收集65例胃良性病变患者正常胃黏膜组织(正常组织),采用免疫组织化学方法检测各组织FBP1蛋白表达,实时定量聚合酶链反应(RT-PCR)检测组织FBP1 mRNA表达;收集胃癌患者临床病理资料,分析FBP1表达水平与胃癌病理资料的关系;对胃癌患者进行随访,分析FBP1表达与患者生存率的关系。结果:胃癌组织FBP1蛋白高表达率为27.2%(22/81),癌旁组织为56.8%(46/81),正常组织为83.1%(54/65),胃癌组织FBP1蛋白高表达率显著低于癌旁组织和正常组织(P<0.05);胃癌组织FBP1蛋白染色评分亦显著低于癌旁组织和正常组织(P<0.05)。FBP1 mRNA相对表达量胃癌组织为(0.294±0.048),癌旁组织为(0.531±0.067),正常组织为(0.935±0.098),胃癌组织显著低于癌旁组织和正常组织(P<0.05)。胃癌组织FBP1表达水平与肿瘤直径、TNM分期、淋巴结转移有关,肿瘤直径越大、TNM分期越高、合并淋巴结转移的胃癌患者FBP1低表达率越高(P<0.05)。胃癌患者FBP1高表达组3年累积生存率为77.4%,FBP1低表达组3年累积生存率为43.8%,FBP1高表达组显著高于低表达组(P<0.05)。结论:胃癌组织FBP1表达显著降低,其表达水平与肿瘤大小、临床分期、淋巴结转移有关;FBP1表达越高,胃癌患者生存率越高,FBP1可以作为胃癌病情和预后的预测指标之一。

Objective: To explore the fructose-1, 6-bisphosphatase 1(FBP1) expression in gastric cancer and its predictive value for patients prognosis. Methods:A total of 81 cases of gastric carcinoma patients gastric mucosa tissue samples(gastric cancer tissue) and adjacent non tumor mucosa epithelial tissue(adjacent issue) were collected and the normal gastric mucosa(normal tissue) of 65 patients with benign gastric lesions was selected,the FBP1 protein expression level was detected by immunohistochemistry,FBP1 mRNA expression level was detected by RT-PCR.The clinical pathological data of gastric cancer patients was collected,and the relationship between the expression level of FBP1 and the pathological data of the patients with gastric carcinoma were analysed.Gastric cancer patients were followed up, the relationship between the expression of FBP1 and the survival rate of patients were analysed. Results: Gastric cancer tissue FBP1 protein high expression rate was 27.2%(22/81),cancer adjacent tissues was 56.8%(46/81), normal tissue was 83.1%(54/65), gastric cancer tissues FBP1 protein high expression rate was significantly lower than that in the tumor adjacent tissues and normal tissues(P<0.05),the staining score of FBP1 protein in gastric carcinoma tissue was significantly lower than that in adjacent tissues and normal tissues(P<0.05).FBP1 mRNA relative expression in gastric cancer tissues was(0.294±0.048),cancer adjacent tissues was(0.531±0.067),normal tissue was(0.935±0.098),FBP1 mRNA in gastric cancer tissue relative expression was significantly lower than that in paracancerous tissues and normal tissues(P<0.05).The expression level of FBP1 in gastric cancer was related to tumor diameter, TNM stage, lymph node metastasis,the larger the tumor diameter, the higher the TNM stage, and with lymph node metastasis patients lower FBP1 expression rate was higher(P<0.05).The 3 year cumulative survival rate of FBP1 patients with high expression in gastric cancer was 77.4%, 43.8% in low FBP1 expression patients,the 3 year cumulative survival rate of FBP1 high expression group was significantly higher than that in low expression group(P<0.05). Conclusion: The expression level of FBP1 in gastric carcinoma is significantly decreased,the expression level of FBP1 is relate to tumor size, clinical stage and lymph node metastasis.The higher FBP1 expression, the higher survival rate of patients with gastric cancer, FBP1 can be used as one of the prognosis indicators of prognosis for gastric cancer.

参考文献:

[1] 邹小农,孙喜斌,陈万青,等.2003-2007年中国胃癌发病与死亡情况分析[J].肿瘤,2012,32(2):109-114.
[2] 雷星,金犇,杨薇粒,等.中国内陆地区恶性肿瘤家族史与胃癌发病风险的meta分析[J].山西医科大学学报,2015,46(9):899-904.
[3] RIZZELLO C G,NIONELLI L,CODA R,et al.Synthesis of the cancer preventive peptide lunasin by lactic acid bacteria during sourdough fermentation[J].Nutr Cancer,2012,64(1):111-120.
[4] REDDY M M,FERNANDES M S,DESHPANDE A,et al.The JAK2V617F oncogene requires expression of inducible phosphofructokinase/fructose-bisphosphatase 3 for cell growth and increased metabolic activity[J].Leukemia,2012,26(3):481-489.
[5] 吴二斌,李莉华,郭子健,等.PFKFB3在肝癌中的表达及其临床意义[J].临床肿瘤学杂志,2014(6):508-511.
[6] CHEN M,ZHANG J,LI N,et al.Promoter hypermethylation mediated downregulation of FBP1 in human hepatocellular carcinoma and colon cancer[J].PLoS One,2011,6(10):e25564.
[7] TANG X,LAN Z,CHEN Y,et al.The 7th AJCC/UICC TNM staging system may be not suitable in predicting prognosis of synchronous multiple gastric carcinoma patients with D2 gastrectomy[J].Tumour Biol,2015,36(5):3653-3659.
[8] 冯鑫,刘畅,钟殿胜,等.免疫组化染色评分对EGFR突变检测的影响[J].中国肺癌杂志,2015(12):740-744.
[9] ZAIZEN Y,AZUMA K,KURATA S,et al.Prognostic significance of total lesion glycolysis in patients with advanced non-small cell lung cancer receiving chemotherapy[J].Eur J Radiol,2012,81(12):4179-4184.
[10] GRINDE M T,MOESTUE S A,BORGAN E,et al.(13)C High-resolution-magic angle spinning MRS reveals differences in glucose metabolism between two breast cancer xenograft models with different gene expression patterns[J].NMR Biomed,2011,24(10):1243-1252.
[11] 许晓巍,孟祥军,王椿,等.糖酵解与肿瘤[J].国际肿瘤学杂志,2011,38(8):585-589.
[12] DONG C,YUAN T,WU Y,et al.Loss of FBP1 by snail-mediated repression provides metabolic advantages in basal-like breast cancer[J].Cancer Cell,2013,23(3):316-331.
[13] 张海涛,郭雅,曹成明,等.1,6-二磷酸果糖在肿瘤诊疗中的应用进展[J].广东医学,2013,34(9):1451-1453.
[14] 杨璟,陈建平,徐斌,等.果糖-1,6-二磷酸酶在肝癌组织的表达及其对预后的影响[J].中华实验外科杂志,2016,33(4):1088-1090.
[15] RABENHORST U,BEINORAVICIUTE-KELLNER R,BREZNICEANU M L,et al.Overexpression of the far upstream element binding protein 1 in hepatocellular carcinoma is required for tumor growth.[J].Hepatology,2009,50(4):1121-1129.
[16] WANG B,HSU S H,FRANKEL W,et al.Stat3-mediated activation of microRNA-23a suppresses gluconeogenesis in hepatocellular carcinoma by down-regulating Glucose-6-phosphatase and peroxisome proliferator-activated receptor gamma,coactivator 1 alpha[J].Hepatology,2012,56(1):186-197.
[17] LI B,QIU B,LEE D S,et al.Fructose-1,6-bisphosphatase opposes renal carcinoma progression[J].Nature,2014,513(7517):251-255.
[18] TAYYEM R F,ZALLOUM H M,ELMAGHRABI M R,et al.Ligand-based designing,in silico screening,and biological evaluation of new potent fructose-1,6-bisphosphatase(FBPase) inhibitors[J].Eur J Med Chem,2012,56:70-95.
[19] BOBARYKINA A Y,MINCHENKO D O,OPENTANOVA I L,et al.Hypoxic regulation of PFYFB-3 and PFKFB-4 gene expression in gastric and pancreatic cancer cell lines and expression of PFYFB genes in gastric cancers[J].Acta Biochim Pol,2006,53(4):789-799.
[20] 彭秋平,梁后杰.糖酵解代谢在恶性肿瘤细胞中的特异性表型及其意义[J].临床肿瘤学杂志,2009,14(5):470-473.
[21] 王千千,关泉林,祝秉东,等.肿瘤细胞糖酵解途径的抗肿瘤研究[J].国际肿瘤学杂志,2011,38(12):914-916.

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