Objective: To explore the expression of Kif2a in human epithelial ovarian cancer cells, and the influences of Kif2a gene silencing on cancer cell proliferation, invasion and apoptosis. Methods: Human epithelial ovarian cancer cell lines (OVCAR-3, SKOV-3) and human normal ovarian epithelial cell line (IOSE-80) were routinely cultured. The levels of Kif2a mRNA and protein in cells were detected by qRT-PCR and Western blots, respectively. Kif2a gene in OVCAR-3 and SKOV-3 cells was silenced by siRNA-interference, which was verified by qRT-PCR and Western blots. Cell proliferation was measured by MTT assay. Cell invasion ability was determined by Transwell assay. Flow cytometry was used to detect cell apotosis rate. The expression of apottosis-related proteins (active-caspase 3 and Bcl-2) were detected by Western blot.Results: The expression of Kif2a in cancer cells was significantly higher than that in normal cells. After silencing Kif2a gene, the proliferation and invasion abilities of OVCAR-3 and SKOV-3 cells were markedly decreased. Oppositely, cell apoptosis rate was dramatically increased with enhanced active-caspase 3 expressions and reduced Bcl-2 expressions.Conclusion: For epithelial ovarian cancer, the expression of Kif2a is aberrantly increased in cancer cells. Silencing of Kif2a gene can dramatically inhibit epithelial ovarian cancer cell proliferation and invasion, but induce cell apoptosis. |
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