Objective: To investigate the effect of extracellular matrix (ECM) on the differentiation,colonization and survival of cardiac muscle derived stem cells (CDC) in rats with acute myocardial infarction. Methods: Female Wistar rats were used to establish acute myocardial infarction model and the CDC and ECM were prepared by the method of myocardial tissue block and cell free. The CDC in IC group (n=6) were cultured with Toure Beko medium (IMDM) modified by Iscoves, and the final concentration of culture was 1×106·(150 μl)-1. The CDC in EC group (n=6) were cultured within medium, and the final concentration of culture was 1×106·(150 μl)-1. The expressions of vascular endothelial cell (vWF),myocardial cells (α-SA),smooth muscle cells (α-SMA) of the two groups after 24 h and 3 weeks of implantation CDC were detected.Results: The gray ratio of α-SA,VWF and α-SMA gray ratio,CT value of 24 h,3 weeks after myocardial infarction (SRY gene) in the IC group were significantly different from those in the EC group (P<0.05)[(0.46±0.06) vs.(0.83±0.03),(0.15±0.08) vs.(0.31±0.01),(0.18±0.11)vs.(0.5±0.07),(1.89±0.67) vs.(5.58±1.44),(0.5±0.26)vs.(2.18±1.28),respectively].Conclusion: Myocardial tissue derived ECM can promote the differentiation of CDC in myocardium,vascular endothelial cells and smooth muscle cells and promote the implantation and survival of CDC after myocardial infaurvival |
[1] DAI W,GERCZUK P,ZHANG Y,et al.Intramyocardial injection of heart tissue-derived extracellular matrix improves postinfarction cardiac function in rats[J].J Cardiovasc Pharmacol Ther,2013,18(3):270-279.
[2] PARAMESWARAN S,KUMAR S,VERMA R S,et al.Cardiomyocyte culture——an update on the in vitro cardiovascular model and future challenges[J].Can J Physiol Pharmacol,2013,91(12):985-998.
[3] MALLIARAS K,MAKKAR R R,SMITH R R,et al.Intracoronary cardiosphere-derived cells after myocardial infarction:evidence of therapeutic regeneration in the final 1-year results of the CADUCEUS trial[J].J Am Coll Cardiol,2014,63(2):110-122.
[4] SMITH R R,BARILE L,CHO H C,et al.Regenerative potential of cardiosphere-derived cells expanded from percutaneous endomyocardial biopsy specimens[J].Circulation,2007,115(56):896-908.
[5] MALLIARAS K,LI T S,LUTHRINGER D,et al.Safety and efficacy of allogeneic cell therapy in infarcted rats transplanted with mismatched cardiosphere-derived cells[J].Circulation,2012,125(7):100-112.
[6] CHENG K,LI T S,MALLIARAS K,et al.Magnetic targeting enhances engraftment and functional benefit of iron-labeled cardiosphere-derived cells in myocardial infarction[J].Circ Res,2010,106(10):1570-1581.
[7] 唐丽.组织特异性细胞外基质对体外培养hPDLSCs干细胞特性的维持及体内分化的研究[D].西安:第四军医大学,2014.
[8] 姜大朋,李岩,杨墨文,等.大鼠皮肤成纤维细胞分泌的细胞外基质对肌腱干细胞特性的影响[J].中国矫形外科杂志,2014,22(15):1408-1411.
[9] DAVIS D R,RUCKDESCHEL S R,MARBAN E.Human cardiospheres are a source of stem cells with cardiomyogenic potential[J].Stem Cells,2010,28(5):903-904.
[10] 姜大庆,谷天祥,徐兆发,等.心肌细胞外基质对心肌球源性心肌干细胞体外分化、增殖及凋亡影响的研究[J].中国胸心血管外科临床杂志,2016,23(9):901-906.
[11] 姜大庆,谷天祥,徐兆发,等.心肌球源性心肌干细胞联合心室肌细胞外基质治疗大鼠急性心肌梗死效果[J].南方医科大学学报,2016,36(10):1316-1321.
[12] GAETANI R,LEDDA M,BARILE L,et al.Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields[J].Cardiovasc Res,2009,82(3):411-420.
[13] 许小媛.大鼠骨髓基质干细胞向心肌细胞的诱导分化与移植治疗心肌梗死的实验研究[D].扬州:扬州大学,2011. |
