凋亡诱导因子在缺氧缺血性脑病中的表达及其与疾病预后的关系-现代医学

凋亡诱导因子在缺氧缺血性脑病中的表达及其与疾病预后的关系

单位：徐州市第九七医院神经内科, 江苏徐州 221004

分类号：R34

出版年·卷·期（页码）：2017·36·第五期（695-698）

摘要：

目的：检测缺氧缺血性脑病患者血清中凋亡诱导因子水平，分析其与疾病预后的关系。方法：选取在我院就诊的72例缺氧缺血性脑病患者作为研究对象，以同期体检的健康者50例为对照。采集研究对象的血液，PCR及ELISA法检测血清中凋亡诱导因子水平，并分析其与疾病预后的关系。结果：凋亡诱导因子表达水平研究组与对照组比较，差异均有统计学意义（P < 0.05）。1个月未治愈者凋亡诱导因子表达水平与1个月治愈者比较，差异均有统计学意义（P < 0.05）。治疗2、4个月后回访显示，存在后遗症的患者治疗后1个月凋亡诱导因子表达水平与无后遗症者比较，差异均有统计学意义（P < 0.05）。结论：凋亡诱导因子水平高的缺氧缺血性脑病患者治愈所需时间更长，有后遗症的患者凋亡诱导因子水平表达更高。

Objective: To detect the level of apoptosis-inducing factor in patients with hypoxic-ischemic encephalopathy, and to analyze the relationship between the level of apoptosis inducing-factor and the prognosis of the disease.Methods: Seventy-two patients with hypoxic-ischemic encephalopathy were enrolled in this study(study group) and 50 healthy subjects were selected as controls. The serum of the subjectswas collected and the level of apoptosis-inducing factor was detected by PCR and ELISA. The relationship between the levels of apoptosis-inducing factor and the prognosis was also analyzed. Results: The expression of apoptosis-inducing factor was significantly different between the study group and the control group (P<0.05).The expression of apoptosis-inducing factor in the patients without cure after 1 month treatment was significantly different from the patients cured after 1 month treatment (P<0.05). 2,4-months follow-up showed that the expression level of apoptosis-inducing factors in patients with sequelae was significantly different from that in patients without sequelae (P<0.05). Conclusion: Patients with hypoxic-ischemic encephalopathy with a high level of apoptosis-inducing factor have a longer cure time and a higher level of apoptosis-inducing factor in patients with sequelae.

参考文献：

[1] 林少娟,孙凯华,黄梅秀,等.不同疗程高压氧治疗对缺血缺氧性脑病患儿日常生活能力的影响[J].广东医学,2013,34(13):2046-2048.
[2] PLESNILA N,ZHU C,CULMSEE C,et al.Nuclear translocation of apoptosis-inducing factor after focal cerebral ischemia[J].J Cereb Blood Flow Metab,2004,24(4):458-466.
[3] SUSIN S A,DAUGUS E,RAVAGNAN L,et al.Two distinct pathways leading to nuclear-apoptosis[J].J Exp Med,2000,192(4):571-580.
[4] BABU C S,RAMANATHAN M.Post-ischemic administration of nimodipine following focal cerebral ischemic-reperfusion injury in rats alleviated excitotoxicity,neurobehavioural alterations and partially the bioenergetics[J].Int J Devl Neuroscience,2011,29(1):93-105.
[5] 王良,余丹.成人重症缺血缺氧性脑病34例CT、MRI分析[J].中国现代医学杂志,2008,18(13):1889-1890,1893.
[6] 黄小玲,肖听,李思涛,等.磁共振扩散加权成像的表观弥散系数在评估新生儿缺氧缺血性脑病中的价值[J].实用儿科临床杂志,2008,23(17):1361-1363.
[7] JOZA N,SUSIN S A,DAUGUS E,et al.Essential role of the mitochondrial apoptosis-inducing factor in programed cell death[J].Nature,2001,410(6828):549-554.
[8] CANDE C,COHEN I,DAUGAS E,et al.Apoptosis-inducing factor(AIF):A novel caspase-independent death efector released from mitochondria[J].Biochimic,2002,84(2-3):215-222.
[9] DELETTRE C,YUSTE V J,MOUBARAK R S,et al.AIFsh,a novel apoptosis-inducing factor(AIF) pro-apoptotic isoform with potential pathological relevance in human cancer[J].J Biol Chem,2006,281(10):6413-6427.
[10] CAO G,CLARK R S,PEI W.Translocation of apoptosis-inducing factor in vulnerable neurons after transient cerebral ischemia and in neuronal cultures after oxygen-glucose deprivation[J].J Cereb Blood Flow Metab,2003,23(10):1137-1150.
[11] SENDOEL A,HENGARTNER M O.Apoptotic cell death under hypoxia[J].Physiology(Bethesda),2014,29(3):168-176.
[12] 张丽,苏志强,陈丽霞.亚低温通过抑制两种凋亡通路对大鼠脑缺血再灌注损伤发挥保护作用[J].中风与神经疾病杂志,2006,23(3):318-321.

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