HMGB1对胆囊癌细胞增殖、凋亡的影响及其机制研究-现代医学

HMGB1对胆囊癌细胞增殖、凋亡的影响及其机制研究

单位：上海市徐汇区大华医院外科, 上海 200237

分类号：R735.8

出版年·卷·期（页码）：2017·36·第十期（1422-1426）

摘要：

目的：探讨高迁移率蛋白1（HMGB1）对胆囊癌细胞增殖、凋亡的影响及其机制。方法：胆囊癌细胞GBC-SD转染HMGB1小干扰RNA（HMGB1 siRNA）、siRNA阴性对照，分别记为干扰组和siRNA-NC组，以不转染的细胞为对照组。RT-PCR和蛋白质印迹法分别检测转染效果，噻唑蓝（MTT）法检测细胞增殖，流式细胞仪检测细胞凋亡，蛋白质印迹法检测β-连环蛋白、c-myc和活化的含半胱氨酸的天冬氨酸蛋白水解酶3的表达。结果：对照组和siRNA-NC组细胞中HMGB1 mRNA和蛋白表达水平[光密度值（OD值）]、细胞凋亡率、β-连环蛋白、c-myc和活化的含半胱氨酸的天冬氨酸蛋白水解酶3表达水平没有明显变化。干扰组HMGB1 mRNA和蛋白表达水平及β-连环蛋白、c-myc表达水平明显低于对照组，而细胞凋亡率明显高于对照组，差异具有统计学意义（P<0.05）。结论：干扰HMGB1可能通过调控Wnt信号通路抑制胆囊癌细胞增殖，促进胆囊癌细胞凋亡。

Objective: To investigate the effect of HMGB1 on proliferation and apoptosis of gallbladder cancer cells and its mechanism. Methods: The gallbladder cancer cell GBC-SD transfected with HMGB1 siRNA and siRNA control was used as interference group and siRNA -NC group, and the non transfected cells were served as control group. The transfection efficiency was monitored by RT-PCR and Western blot, the cell proliferation and apoptosis were respectively detected by MTT and flow cytometry, and the expressions of β-catenin, c-myc and Cleaved Caspase-3 were detected by Western blot. Results: No significant changes of HMGB1 mRNA and protein, β-catenin, c-myc and Cleaved Caspase-3 levels(OD value), apoptosis rate were found in siRNA-NC group compared with those in the control group.HMGB1 mRNA and protein, β-catenin and c-myc in the interference group were obviously decreased compared with those in the control group, while the apoptosis rate and Cleaved Caspase-3 were obviously increased, the difference being statistically significant(P<0.05). Conclusion: Interference with HMGB1 may inhibit the proliferation of gallbladder cancer cells by regulating the Wnt signaling pathway.

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