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FSH对卵巢癌细胞增殖、侵袭作用的影响及可能的机制
作者:路晓琳 
单位:邯郸市中心医院 妇科, 河北 邯郸 056001
关键词:卵泡刺激素 卵巢癌 细胞增殖 侵袭能力 
分类号:R737.31
出版年·卷·期(页码):2017·36·第十二期(1730-1734)
摘要:

目的:探讨卵泡刺激素(FSH)对卵巢癌细胞增殖、侵袭作用的影响及可能的机制。方法:(1)将购买自北京大学人民医院妇产科实验室的进入生长对数期的卵巢癌SKOV-3细胞与浓度为0、10、20、40、80 mIU·ml-1的FSH液共培养,同时设立对照组(只加培养基,不加FSH),采用MMT法观察不同作用时间各组细胞的增殖情况;(2)将SKOV-3细胞分为空白组和FSH (40 mIU·ml-1)干预组共培养48 h,Wester-blotting法检测各组中FSH受体(FSHR)、磷酸化蛋白激酶B (pAKT)、E-cadherin蛋白的表达水平,采用Transwell小室测定细胞侵袭转移能力。结果:在共培养12、24、48、72 h时间点,FSH浓度10、20、40、80 mIU·ml-1组的SKOV-3细胞增殖率显著高于0 mIU·ml-1组(P<0.05);FSH浓度40 mIU·ml-1组的SKOV-3细胞增殖率在共培养12、24、48、72 h时间点均显著高于FSH浓度10、20、80 mIU·ml-1组(P<0.05),且在共培养48 h时达最大值;在共培养48 h后,FSH干预组的SKOV-3细胞上清液中pAKT蛋白水平显著高于空白组(P<0.05),E-cadherin蛋白水平显著低于空白组(P<0.05),两组间SKOV-3细胞上清液中FSHR蛋白表达水平差异无统计学意义(P>0.05);在共培养48 h后,FSH干预组的SKOV-3细胞穿过基质膜的平均细胞数显著高于空白组(P<0.05)。结论:FSH能促进卵巢癌细胞增殖,提高其侵袭能力,作用机制与降低E-cadherin蛋白水平、提高pAKT蛋白表达有关。

Objective: To investigate the mechanism of follicle stimulating hormone (FSH) in promoting the proliferation and invasion of ovarian cancer cells.Methods: (1) SKOV-3 cells purchased from the Department of Obstetrics and Gynecology of Peking University People's Hospital Laboratory entered the logarithmic growth phase were divided into 0, 10, 20, 40, 80 mIU·ml-1 FSH coculture group. MMT method was used to observed the proliferation of cells in thegroups at different action time; (2) SKOV-3 cells were divided into control group and FSH (40 mIU·ml-1) intervention group, after cocultured 48 h, FSH receptors (FSHR), phosphorylated protein kinase B (pAKT), the expression level of E-cadherin protein were detected by Wester-blotting. Cell invasion and metastasis were determined by Transwell.Results: At the time of cocultured 12, 24, 48 and 72 h, the proliferation rate of SKOV-3 cells was significantly higher inFSH concentration 10, 20, 40 and 80 mIU·ml-1 four groups than in 0 mIU·ml-1 group (P<0.05). The cell proliferation rate of SKOV-3 cells in FSH 40 mIU·ml-1 group at cocultured 12, 24, 48, 72 h time points were significantly higher than those of the FSH 10, 20 and 80 mIU·ml-1 groups (P<0.05), and the maximumwas reached at cocultured48 h. After cocultured 48 h, the level of pAKT protein in the supernatant of SKOV-3 cells in FSH intervention group was significantly higher than that in the blank group (P<0.05); the level of E-cadherin protein was significantly lower than that of the blank group (P<0.05); there was no significant difference in the expression of FSHR protein in the supernatant of SKOV-3 cells between the two groups (P>0.05). After cocultured 48 h, the average number of SKOV-3 cells passing through the stroma membrane in the FSH intervention group was significantly higher than that in the blank group (P<0.05).Conclusion: FSH can promote the proliferation and invasion of ovarian cancer cells, the mechanism is reducing the level of E-cadherin protein and improving the expression of pAKT protein.

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