Objective: To investigate the mechanism of follicle stimulating hormone (FSH) in promoting the proliferation and invasion of ovarian cancer cells.Methods: (1) SKOV-3 cells purchased from the Department of Obstetrics and Gynecology of Peking University People's Hospital Laboratory entered the logarithmic growth phase were divided into 0, 10, 20, 40, 80 mIU·ml-1 FSH coculture group. MMT method was used to observed the proliferation of cells in thegroups at different action time; (2) SKOV-3 cells were divided into control group and FSH (40 mIU·ml-1) intervention group, after cocultured 48 h, FSH receptors (FSHR), phosphorylated protein kinase B (pAKT), the expression level of E-cadherin protein were detected by Wester-blotting. Cell invasion and metastasis were determined by Transwell.Results: At the time of cocultured 12, 24, 48 and 72 h, the proliferation rate of SKOV-3 cells was significantly higher inFSH concentration 10, 20, 40 and 80 mIU·ml-1 four groups than in 0 mIU·ml-1 group (P<0.05). The cell proliferation rate of SKOV-3 cells in FSH 40 mIU·ml-1 group at cocultured 12, 24, 48, 72 h time points were significantly higher than those of the FSH 10, 20 and 80 mIU·ml-1 groups (P<0.05), and the maximumwas reached at cocultured48 h. After cocultured 48 h, the level of pAKT protein in the supernatant of SKOV-3 cells in FSH intervention group was significantly higher than that in the blank group (P<0.05); the level of E-cadherin protein was significantly lower than that of the blank group (P<0.05); there was no significant difference in the expression of FSHR protein in the supernatant of SKOV-3 cells between the two groups (P>0.05). After cocultured 48 h, the average number of SKOV-3 cells passing through the stroma membrane in the FSH intervention group was significantly higher than that in the blank group (P<0.05).Conclusion: FSH can promote the proliferation and invasion of ovarian cancer cells, the mechanism is reducing the level of E-cadherin protein and improving the expression of pAKT protein.
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