目的：研究SHIP2蛋白在乳腺癌组织、乳腺癌旁正常组织、乳腺癌患者淋巴结中的表达情况，探讨SHIP2在乳腺癌发生发展过程中的可能作用及其临床意义以及SHIP2与乳腺癌淋巴结转移的相关性。方法：应用Western blotting和RT-PCR检测60例乳腺癌及癌旁组织中SHIP2蛋白及SHIP2 mRNA表达情况。应用免疫组织化学法检测50例淋巴结阴性转移组（A组）中的淋巴结、10例淋巴结阳性转移组（B组）中的转移淋巴结中SHIP2蛋白表达情况。结果：乳腺癌组织中SHIP2蛋白表达量明显高于癌旁正常乳腺组织（P<0.05）；乳腺癌组织中SHIP2 mRNA表达量明显高于癌旁乳腺组织（P<0.05）；SHIP2蛋白在B组淋巴结中阳性表达率高于A组，差异有统计学意义（P<0.05）。结论：SHIP2过表达与乳腺癌密切关联，其可能成为防控乳腺癌的靶点。SHIP2过表达可能与乳腺癌淋巴结转移有正性相关性。

[1] 李梅芳,何建蓉,沈坤炜,等.不同年龄段乳腺癌患者的临床病理特点[J].中华乳腺病杂志:电子版,2010,4(5):558-565.
[2] SASAOKA T,WADA T,TSUNEKI H.Lipid phosphatases as a possible therapeutic target in cases of type 2 diabetes and obesity[J].Pharmacol Ther,2006,112(3):799-809.
[3] SLEEMAN M W,WORTLEY K E,LAI K M,et al.Absence of the lipid phosphatase SHIP2 confers resistance to dietary obesity[J].Nat Med,2005,11(2):199-205.
[4] ZHUANG G,HUNTER S,HWANG Y,et al.Regulation of EphA2 receptor endocytosis by SHIP2 lipid phosphstase via phosphatidylinositol3-Kinase-dependent Racl activation[J].J Biol Chem,2007,282(4):2683-2694.
[5] BLERO D,PAYRASTRE B,SCHURMANS S,et al.Phosphoinositide phosphatases in a network of signalling reactions[J].Pflugers Arch,2007,455:31-44.
[6] WENDEL H,de STANCHINA E,FRIDMAN J,et al.Survival signaling by Akt and eIF4E in oncogenesis and cancer therapy[J].Nature,2004,428:332-337.
[7] GIURIATO S,BLERO D,ROBAYE B,et al.SHIP2 overexpression strongly reduces the proliferation rate of K562 erythroleukemia cell line[J].Biochem Biophys Res Commun,2002,296:106-110.
[8] PESESSE X,DELEU S,DeSMEDT F,et al.Identification of a second SH2-domain-containing protein closely related to the phosphatidylinositol polyphosphate 5-phosphatase SHIP[J].Biochem Biophys Res Commun,1997,239(3):697-700.
[9] SUMIE S,KAWAGUCHI T,KOMUTA M,et al.Significance of glucose intolerance and SHIP2 expression in hepatocellular carcinoma patients with HCV infection[J].Oncol Rep,2007,18(3):545-552.
[10] 葛燕梅,叶艳,张林杰,等.SHIP2通过上调Bim表达增强胃癌细胞BGC-823对紫杉醇的敏感性[J].中国药理学通报,2014,30(5):701-706.
[11] ZHOU X,LIU Y,TAN G.Prognostic value of elevated SHIP2 expression in laryngeal squamous cell carcinoma[J].Arch Med Res,2011,42(7):589-595.
[12] 周小娟,张俊杰,谭国林,等.SHIP2在喉鳞状细胞癌中的表达及意义[J].中国耳鼻咽喉颅底外科杂志,2011,17(5):321-326.
[13] PRASAD N K,TANDON M,HANDA A,et al.High expression of obesity-linked phosphatase SHIP2 in invasive breast cancer correlates with reduced disease-free survival[J].Tumour Biol,2008,29(5):330-341.
[14] PRASAD N K,TANDON M,BADVE S,et al.Phosphoinositol phosphatase SHIP2 promotes cancer development and metastasis coupled with alterations in EGF receptor turnover[J].Carcinogenesis,2008,29(1):25-34.
[15] PRASAD N K.SHIP2 phosphoinositol phosphatase positively regulates EGFR-Akt pathway,CXCR4 expression,and cell migration in MAD-MDA-MB-231 breast cancer cells[J].Int Joncle,2009,34(1):97-105.