目的：采用高效液相色谱串联质谱（LC-MS/MS）法检测不明原因复发性流产患者（URSA）干血斑中多种氨基酸含量，探讨血中氨基酸水平的变化与URSA的关系。方法：采用API 3200型LC-MS/MS检测35例URSA患者和20例正常早孕妇女干血斑中11种氨基酸水平，比较两者的差异。结果：URSA患者干血斑中被检氨基酸（除精氨酸外）水平明显高于正常早孕妇女（P<0.05）。结论：LC-MS/MS法可用于检测不明原因复发性流产患者血中氨基酸谱的变化，URSA患者血中氨基酸水平有明显的变化。

[1] 马韵,郑梅玲.不明原因复发性流产发病机制研究进展[J].中华妇幼临床医学杂志:电子版,2016,12(2):237-240.
[2] 陈嵩,任涛,万健,等.慢阻肺患者血清炎性指标、血浆及尿液游离氨基酸的变化研究[J].实用临床医药杂志,2017,21(3):22-24.
[3] THOMAS J W,MARTIN G L,RAMACHANDRAN S V,et al.Metabolite profiles and the risk of developing diabetes[J].Nat Med,2011,17(4):448-453.
[4] INDIRA R,HERALD J,PRATIBHA R,et al.Amino acid profile of saliva from patients with oral squamous cell carcinoma using high performance liquid chromatography[J].J Oral Sci,2012,54(3):279-283.
[5] 辛静,黄金,李真.妊娠期糖脂及氨基酸代谢的研究进展[J].西部医学,2011,23(5):982-984.
[6] 易艳红,董梅,陈创奇,等.胚胎培养液中氨基酸浓度对选择性单胚胎移植妊娠结局的影响[J].中华实用诊断与治疗杂志,2014,28(12):1163-1165.
[7] STOKES P J,HAWKHEAD J A,FAWTHROP R K,et al.Metabolism of human human embryos following cryopreservation:implications for the safety and selection of embryos for transfer in clinical IVF[J].Hum Rep,2007,22(3):829-835.
[8] von VRESEN-HOEYNCK F M,POWERS R M.Maternal-fetal metabolism in normal pregnancy and preeclampsia[J].Front Biosci,2007,12(7):2457-2470.
[9] CAMELO J S Jr,JORGE S M,MARTINEZ F E.Amino acid composition of parturient plasma,the intervillous space of the placenta and the umbilical vein of term new born infants[J].Braz J Med Biol Res,2004,37(5):711-717.
[10] DIAZ M,BASSOLS J,LOPEZ-BERMEJO A,et al.Placental expression of peroxisome proliferator-activated receptor γ(PPARγ):relation to placental and fetal growth[J].J Clin Endocrinol Metab,2012,97(8):E1468-1472.