骨髓间充质干细胞miR-126高表达在游离皮瓣移植后皮瓣血管新生中的促进作用及机制研究-现代医学

骨髓间充质干细胞miR-126高表达在游离皮瓣移植后皮瓣血管新生中的促进作用及机制研究

单位：1. 湖北医药学院附属人民医院烧伤整形外科, 湖北十堰 442000;
2. 湖北医药学院附属东风医院血液内科, 湖北十堰 442000

分类号：R392;R622

出版年·卷·期（页码）：2018·46·第二期（126-130）

摘要：

目的：研究骨髓间充质干细胞（BMSCs）microRNA-126（miR-126）高表达在游离皮瓣移植后皮瓣新生血管中的促进作用及机制。方法：将24只SD大鼠随机分为观察组分别在游离皮瓣远端1 cm与3 cm处注射2 ml含有1×10⁶ miR-126 mimics转染的BMSCs，阴性对照组为注射miR-126 mimics control转染的BMSCs，空白对照组注入等量的磷酸盐缓冲液，三组各8只。通过瞬时转染的方法增强BMSCs中miR-126的表达水平，对三组皮瓣组织液中的TNF-α、MIP-1α和VCAM-1蛋白水平进行测定，并检测三组BMSCs中TNF-α、MIP-1α和VCAM-1 mRNA的表达水平。结果：观察组皮瓣组织液中TNF-α、MIP-1α和VCAM-1蛋白表达水平较空白对照组、阴性对照组均显著降低（均P<0.01），而阴性对照组TNF-α、MIP-1α和VCAM-1蛋白表达水平较空白对照组均显著降低（均P<0.01）。观察组转染后BMSCs中TNF-α、MIP-1α和VCAM-1 mRNA相对表达量较空白对照组、阴性对照组均显著降低（均P<0.01），而阴性对照组TNF-α、MIP-1α和VCAM-1 mRNA相对表达量较平较空白对照组均显著降低（均P<0.01）。结论：在游离皮瓣移植后，miR-126高表达可降低TNF-α、MIP-1α和VCAM-1 mRNA表达水平，使之发挥阻滞血管炎症反应的作用，诱导游离皮瓣组织中血管新生腺管蛋白活化，最终有助于皮瓣新生血管的生成，促进皮瓣成活。

Objective: To study the promoting effect and mechanism of microRNA-126 (miR-126) overexpression in bone mesenchymal stem cells (BMSCs) on angiogenesis after skin flap transplantation. Methods: 24 SD rats were randomly divided into the observation group respectively in the free skin flap of 1 cm and 3 cm injected with 2 ml containing 1×10⁶ miR-126 mimics transfected BMSCs, negative control group was transfected by injection of miR-126 mimics control of BMSCs and the blank control group was injected with phosphate buffer, with 8 rats in each group. The transient transfection was used to enhance the expression level of miR-126 in BMSCs. The protein levels of TNF-α, MIP-1α and VCAM-1 in the flap tissue were determined of the two groups, and the expression levels of TNF-α, MIP-1α and VCAM-1 mRNA in BMSCs were detected in the two groups. Results: The expression levels of TNF-α, MIP-1α and VCAM-1 in the tissue fluid of the observation group were significantly lower than those in the blank control group and the negative control group (all P<0.01), while the expression levels of TNF-α, MIP-1α and VCAM-1 in the negative control group were significantly lower than those in the blank control group (all P<0.01). The observation group after transfection in BMSCs, TNF-α MIP-1α and VCAM-1 mRNA relative expression were significantly lower than those in the blank control group and the negative controlgroup (all P<0.01), and the TNF-α MIP-1α and VCAM-1 mRNA relative expression in the negative control group were significantly lower than those in the blank control group (all P<0.01). Conclusion: After the transplantation of free flap, high expression of miR-126reduces the expression of TNF-α, MIP-1α and VCAM-1 mRNA, which can play the role of blocking vascular inflammation, activating angiogenesis induced by free flap glandular tissue, ultimately contribute to the neovascularization of flaps, and the survival of skin flaps.

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