前列腺癌组织GRP78和GRIM-1表达及其相关性分析-现代医学

前列腺癌组织GRP78和GRIM-1表达及其相关性分析

单位：湖北省天门市第一人民医院手术室, 湖北天门 431700

分类号：R737.25

出版年·卷·期（页码）：2018·46·第二期（162-167）

摘要：

目的：探讨前列腺癌组织葡萄糖调节蛋白78（GRP78）和细胞凋亡相关基因-1（GRIM-1）表达及其与预后的关系。方法：采用免疫组织化学染色检测80例前列腺癌和50例良性前列腺增生组织GRP78、GRIM-1表达，比较GRP78、GRIM-1与前列腺癌患者临床资料的关系；Kaplan-Meier检验分析不同GRP78、GRIM-1表达与前列腺癌患者总体生存率的关系，Cox比例风险模型分析影响患者预后的因素。结果：前列腺癌组GRIM-1蛋白阳性率（90.0%，45/50）明显低于前列腺增生组（67.5%，54/80）（χ²=8.578，P=0.003）；前列腺癌组GRP78蛋白阳性率（95.0%，76/80）明显高于前列腺增生组（76.0%，38/50）（χ²=10.292，P=0.001）。Gleason 2~7分组GRIM-1蛋白阳性率（82.4%，28/34）明显高于Gleason 8~10分组（56.5%，26/46）；Gleason 2~7分组GRP78蛋白阳性率（88.2%，30/34）明显低于Gleason 8~10分组（100.0%，46/46），差异均具有统计学意义（P < 0.05）。前列腺癌组织GRIM-1和GRP78表达呈负相关（r=-0.571，P<0.001）；GRIM-1高表达组总体生存率明显高于低表达组；GRP78高表达组总体生存率明显低于低表达组，差异具有统计学意义（P < 0.05）。多因素分析显示Gleason评分、TNM分期、病理分级、淋巴结转移、GRIM-1和GRP78蛋白表达是影响前列腺癌患者预后的独立因素（均P < 0.05）。结论：前列腺癌组织中GRIM-1、GRP78异常表达；GRIM-1低表达和GRP78高表达与前列腺癌患者不良预后有关。

Objective: To explore the expression of glucose-regulated protein 78 (GRP78) and gene associated with retinoid and interferon-induced mortality 1 (GRIM-1) in prostate cancer tissues and its clinical value for prognositic. Methods: The expression of GRP78 and GRIM-1 in eighty prostate cancer tissues and fifty benign prostatic hyperplasia tissues were performed with immunohistochemical staining, the relationship between GRP78, GRIM-1 and prostate cancer patients clinical data were compared,the over-all survival of patients were analyzed by Kaplan-Meier method, the risk factors influencingthe prognosis of prostate cancer patients were analyzed by Cox proportional hazards model. Results: The positive rate of GRIM-1 protein in the prostate cancer group (90.0%, 45/50) was significantly lower than that in the benign prostatic hyperplasia group (67.5%, 54/80) (χ²=8.578,P=0.003). The positive rate of GRP78 protein in the prostate cancer group (95.0%, 76/80) was significantly higher than that in the benign prostatic hyperplasia group (76.0%, 38/50) (χ²=10.292,P=0.001). The positive rate of GRIM-1 protein in the 2~7 score group (82.4%, 28/34) was significantly higher than in that in the 8~10 score group (56.5%, 26/46), while GRP78 positive rate in the 2~7 score group (88.2%, 30/34) was significantly higher than in the 8~10 score group (100.0%, 46/46), the difference was statistically significant (P<0.05). There was a negative correlation between GRIM-1 and GRP78 in the prostate cancer tissues (r=-0.571, P<0.001). The over-all survival in the GRIM-1 higher expression group was markedly higher than that in the lower expression group, and the over-all survival in the higher GRP78 expression group was markedly lower than that in the lower expression group, the difference was statistically significant (P<0.05). Multivariate analysis showed that Gleason score, TNM stage, pathological grade, lymph node metastasis, GRP78 and GRIM-1 expression were independent risk factors for the poor prognostic of prostate cancer (all P < 0.05). Conclusion: Aberrant expression of GRP78 and GRIM-1 in prostate cancer tissues, the up-regulated of GRP78 and down-regulated of GRIM-1 are risk factors for the poor prognostic of prostate cancer.

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