Objective: To investigate the mechanism of recombinant mutant human tumor necrosis factor-α(rmhTNF-α) inhibit the proliferation of human gastric cancer cell. Methods: Human gastric cancer cell lines MKN45 were treated with different concentrations(0, 50,100,200 U·ml-1) of rmhTNF-α intervention group.The cell inhibition rate was detected by proliferation/toxicity detection kit (CCK-8), and the expression of Δ133p53,cjun,cfos mRNA were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction. Results: The cell inhibition rates in the intervention group was higher than that in the control group(rmhTNF-α 0 U·ml-1 group)and it increased with the increasing of concentration of rmhTNF-α(P<0.05); the expression of Δ133p53 mRNA in the intervention group was lower than that in the control group and it decreased with the increasing of concentration of rmhTNF-α(P<0.05);the expression of cjun,cfos mRNA in the intervention group were higher than those in the control group and they increased with the increasing of concentration of rmhTNF-α(all P<0.05). Pearson correclation analysis showed the expression was negatively correlated markedly betweenΔ133p53 mRNA and cjun,cfos mRNA(r=-0.954,-0.947,respectivly,all P<0.01). Conclusion: rmhTNF may inhibit Δ133p53 expression and promote cjun,cfos expression to further inhibit gastric cancer cell proliferation,the inhibitory effect on Δ133p53 may mediated by cjun and cfos.
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