Objective:To explore the diagnostic value of the serum CA125, G-17, and plasma D-D, M2-PK in patients with gastric cancer.Methods:A total of 70 patients with gastric cancer who were treated in our Hospital from September 2016 to September 2017 were selected as observation group, 45 patients with benign gastric disorders treated in the same period as disease control group, and 45 normal volunteers as health control group. The level and positive rate of serum CA125, G-17, and plasma D-D, M2-PK were compared between the three groups, and the diagnostic efficency of CA125, G-17, D-D and M2-PK for gastric cancer was observed.Results:Serum CA125, G-17, and plasma D-D, M2-PK level were significantly higher in the observation group than thetwo control groups(P<0.05). The levels of CA125, G-17, D-D and M2-PK in different clinical stages were statistically significant(P<0.05). The positive rate of CA125, G-17, D-D and M2-PK were significantly higher in the observation group than that in the two control groups(P<0.05). The sensitivity of combined detection of serum CA125,G-17 and plasma D-D, XM2-PK was significantly higher than that of each alone(P<0.05); however, there was no significant difference in the positive rate between the healthy control group and the disease control group(P>0.05). The specificity of joint detection was lower than each detection alone; when compared with D-D, M2-PK, the difference were statistically significant(P<0.05).Conclusion:Serum CA125,G-17 and plasma D-DX M2-PK have high application value in early diagnosis of gastric cancer, and combined detection of four indexes can significantly improve the sensitivity of diagnosis and avoid missed diagnosis and misdiagnosis to a certain extent. It has important value for early detection of gastric cancer.