Objective:To investigate serum levels of homocysteine (HCY) and glial fibrillary acidic protein (GFAP) in patients with acute cerebral infarction (ACI) and its clinical significance. Methods:Serum HCY and GFAP levels were measured by enzyme-linked immunosorbent assay (ELISA) in 92 patients with ACI and40 patients with non-ACI were included in the control group. The subjects were grouped with the National Institutes of Health Stroke Scale (NIHSS) score, onset time and infarct size. The differences in serum HCY and GFAP levels in different groups were analyzed. The receiver operating characteristic (ROC) curve was constructed to obtain the positive and negative threshold values and diagnostic sensitivity and specificity of HCY and GFAP in the diagnosis of cerebral infarction. Results:The levels of HCY and GFAP in ACI patients were (18.26±5.37) μmol·L-1 and (1.28±0.64) μg·L-1, which were significantly higher than those in the control group (P<0.01). The serum HCY level in the 12-24 h group was higher than the <12 h group (P<0.05). The expression of serum HCY and GFAP was the lowest in the small area infarction (SI) group and the highest in the large area infarction (LI) group, the difference was statistically significant (P<0.01). The NIHSS score <4 group had the lowest HCY and GFAP levels, and the NIHSS sore>15 group was the highest, the difference was statistically significant (P<0.01). There was a positive correlation between serum HCY and GFAPlevels (r=0.514, P=0.001). HCY was positively correlated with hypertension, onset time, infarct size, and NIHSS score. GFAP was positively correlated with hypertension, infarct size, and NIHSS score. Logistic regression analysis found that hypertension, infarct size, and NIHSS score were independent risk factors for serum HCY and GFAP expression levels. ROC analysis showed that the best cutoff value of HCI and GFAP for diagnosis of ACI were 15.03 μmol·L-1, 1.02 μg·L-1. Conclusion:The levels of serum HCY and GFAP in the patients with ACI are significantly increased, and have a certain correlation with the severity of ACI, which can provide a basis for early diagnosis and treatment of ACI.
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