外周血miR-323-3p在异位妊娠早期诊断中的作用-现代医学

外周血miR-323-3p在异位妊娠早期诊断中的作用

单位：1. 湖北省天门市第一人民医院输血科, 湖北天门 431700;
2. 湖北省天门市第一人民医院检验科, 湖北天门 431700

分类号：R446

出版年·卷·期（页码）：2019·47·第八期（900-904）

摘要：

目的：探讨外周血miR-323-3p在异位妊娠早期诊断中的作用。方法：选取我院收治的45例异位妊娠患者与同期45例正常宫内妊娠者进行对比，分别用酶联免疫法、二代测序技术和实时荧光PCR技术检测血清中β-人绒毛膜促性腺激素（HCG）和血浆中miR-323-3p的含量，利用受试者工作曲线（ROC曲线）分析外周血miR-323-3p、β-HCG及二者联合检测对异位妊娠的早期诊断价值，包括曲线下面积、灵敏度和特异度等。结果：异位妊娠组与正常宫内妊娠组在β-HCG 48 h增长率和miR-323-3p相对表达水平方面差异显著（P<0.05），且异位妊娠组有较低的β-HCG 48 h增长率和较高的miR-323-3p相对表达水平；外周血miR-323-3p与β-HCG的检测水平无明显相关性（P>0.05）；外周血miR-323-3p对早期异位妊娠的诊断价值（敏感度86.7%、特异度72.3%）明显高于β-HCG 48 h增长率的诊断价值（敏感度68.6%、特异度70.2%），且当二者联合检测时，诊断价值最高（敏感度91.4%、特异度88.5%）。结论：外周血miR-323-3p对异位妊娠具有较好的早期诊断价值，临床上可以考虑miR-323-3p和β-HCG的联合检测。

Objective:To investigate the diagnostic value of miR-323-3p in early ectopic pregnancy. Methods:A total of 45 cases of ectopic pregnancy patients and 45 normal intrauterine pregnancy women were selected as ectopic pregnancy group and normal pregnancy group, respectively. The levels of serum β-HCG were detected by enzyme linked immunosorbent assay (ELISA), miR-323-3p levels were detected by next-generation sequencing (NGS) and RT-qPCR assay. The diagnostic value of serum β-HCG and miR-323-3p for ectopic pregnancy was determined by ROC curve. Results:The differences were significant in β-HCG 48 h growth rate and miR-323-3p relative expression between the ectopic pregnancy group and the normal pregnancy group (P<0.05). The 48 h growth rate of β-HCG was lower and the relative expression level of miR-323-3p was higher in ectopic pregnancy group compared to the normal pregnancy group. There was no significant correlation between the level of miR-323-3p and β-HCG in peripheral blood (P>0.05). The results of ROC curve analysis showed that the diagnostic value of miR-323-3p in peripheral blood for early ectopic pregnancy was significantly higher than that of β-HCG 48 h growth rate, and the diagnostic value was the highest when the two were combined. Conclusion:Peripheral blood miR-3233p has good early diagnostic value in ectopic pregnancy, and the combined detection of miR-323-3p and β-human chorionic gonadotropin can be considered clinically.

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