目的：探究p120基因对脂多糖（LPS）诱导的支气管上皮细胞炎症因子分泌水平的影响以及调控模式。方法：CCK8法检测0、5、10、20、40、70和100 μg·ml^-1的炎症诱导剂LPS对16HBE细胞存活率的影响。LPS刺激细胞后用Western blotting检测p120蛋白表达水平的变化，RT-qPCR技术检测炎症因子IL-6、TNF-α、IL-1β mRNA的表达水平。采用脂质体法将p120 siRNA转入16HBE细胞后，CCK8法检测细胞增殖情况；RT-qPCR和Western blotting检测siRNA干扰效率和IL-6、IL-1β、TNF-α的表达。结果：LPS呈浓度依赖性抑制16HBE细胞增殖。20 μg·ml^-1的LPS作用于16HBE细胞后，p120表达下调，IL-6、IL-1β、TNF-α表达上调，具有时间依赖性。p120 siRNA对细胞的增殖具有抑制作用，并促进IL-6、IL-1β、TNF-α表达。结论：p120能够抑制LPS诱导的支气管上皮细胞炎症因子的分泌水平。

[1] 张光成,王亚亭.丙酸倍氣米松联合孟鲁司特钠治疗儿童变异性咳嗽及对eotaxin表达水平的影响[J].东南大学学报(医学版),2017,36(2):202-208.
[2] SCHACKMANN R C,TENHAGEN M,VAN DE VEN R A,et al.p120-catenin in cancer- mechanisms,models and opportunities for intervention[J].J Cell Sci,2013,126(16):3515-3525.
[3] GARRETT J P,LOWERY A M,ADAM A,et al.Regulation of endothelial barrier function by p120-catenin·VE-cadherin interaction[J].Mol Biol Cell,2017,28(1):85-97.
[4] YUAN L,ARIKKATHA J.Functional roles of p120ctn family of proteins in central neurons[J].Semi Cell Dev Biol,2017,69(1):70-82.
[5] ZHANG J,RD O J,HOLIAN O,et al.P120 catenin represses transcriptional activity through Kaiso in endothelial cells[J].Microvasc Res,2010,80(2):233-239.
[6] ZHANG Y,ZOU C,YANG S,et al.p120 catenin attenuates the angiotensin II-induced apoptosis of human umbilical vein endothelial cells by suppressing the mitochondrial pathway[J].Int J Mol Med,2016,37(3):623-630.
[7] WANG Y L,MALIK A B,SUN Y,et al.Innate immune function of the adherens junction protein p120-catenin in endothelial response to endotoxin[J].J Immunol,2011,186(5):3180-3187.
[8] 李克彬,唐寅,杨佳奇,等.慢性气道炎症性疾病中细胞因子作用研究进展[J].中国公共卫生,2016,32(4):415-419.
[9] REYNOLDS A B.p120-catenin:past and present[J].Biochim Biophys Acta,2007,1773(1):2-7.
[10] XIAO K,OAS R,CHLASSON C A.Role of p120-catenin in cadherin trafficking[J].Biochim Biophys Acta,2007,1773(1):8-16.
[11] ISHIYAMA N,LEE S H,LIU S,et al.Dynamic and static interactions between p120 catenin and E-cadherin regulate the stability of cell-cell adhesion[J].Cell,2010,141(1):117-128.
[12] ICHII T,TAKEICHI M.p120-catenin regulates microtubule dynamics and cell migration in a cadherin-independent manner[J].Genes Cells,2007,12(7):827-839.
[13] CHEUNG L W,LEUNG P C,WONG A S.Cadherin switching and activation of p120 catenin signaling are mediators of gonadotropin-releasing hormone to promote tumor cell migration and invasion in ovarian cancer[J].Oncogene,2010,29(16):2427-2440.
[14] VAN HENGEL J,VAN ROY F.Diverse functions of p120ctn in tumors[J].Biochim Biophys Acta,2007,1773(1):78-88.
[15] PIETERS T,VAN HENGEL J,VAN ROY F.Functions of p120ctn in development and disease[J].Front Biosci,2012,17(1):760-783.
[16] SMALLEY-FREED W G,EFIMOV A,BURNETT P E,et al.p120-catenin is essential for maintenance of barrier function and intestinal homeostasis in mice[J].J Clin Invest,2010,120(6):1824-1835.
[17] XU H G,GAO Z,MA M M,et al.P120-catenin mediates intermittent cyclic mechanical tension-induced inflammation in chondrocytes[J].J Cell Biochem,2017,118(12):4508-4516.
[18] O'DONNELL J J 3rd,ZHUGE Y,HOLIAN O,et al.Loss of p120 catenin upregulates transcription of pro-inflammatory adhesion molecules in human endothelial cells[J].Microvasc Res,2011,82(2):105-112.
[19] VAGELI D P,DOUKAS S G,SASAKI C.Inhibition of NF-κB prevents the acidic bile-induced oncogenic mRNA phenotype,in human hypopharyngeal cells[J].Oncotarget,2017,9(5):5876-5891.
[20] CATRYSSE L,VAN LOO G.Inflammation and the metabolic syndrome:the tissue-specific functions of NF-κB[J].Trends Cell Biol,2017,27(6):417-429.
[21] QIN S,QIN L,ZHANG C,et al.p120-catenin modulating nuclear factor-κB activation is partially RhoA/ROCK dependent in scratch injury[J].Wound Repair Regen,2015,23(2):231-240.