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结直肠癌组织TRIM58表达、基因甲基化状态及临床意义
作者:尚维伟  王亮  陈信浩 
单位:南京市江宁医院(南京医科大学附属江宁医院) 普外科, 江苏 南京 211100
关键词:TRIM58 结直肠癌 DNA甲基化 病理特征 预后 
分类号:R735.3+5
出版年·卷·期(页码):2020·39·第八期(948-954)
摘要:

目的:探讨结直肠癌组织中三重基序蛋白58(TRIM58)蛋白表达和基因甲基化状态及临床意义。方法:选取95例初治结直肠癌患者的肿瘤组织及配对的癌旁组织标本。采用免疫组化法检测组织TRIM58蛋白表达量,甲基化特异聚合酶链式反应(MSP)检测TRIM58基因启动子区甲基化状态,并分析与患者病理特征及预后的关系。结果:结直肠癌组织中TRIM58蛋白阳性表达率低于癌旁组织[40.0%(38/95)和75.79%(72/95),P<0.05];且与肿瘤直径、临床TNM分期、浸润深度有关(P<0.05)。结直肠癌组织中TRIM58基因甲基化率高于癌旁组织[69.47%(66/95)和35.79%(34/95),P<0.05];且与肿瘤直径、分化程度、淋巴结转移、临床TNM分期、浸润深度有关(P<0.05)。癌旁组织和肿瘤组织中TRIM58基因甲基化状态与TRIM58蛋白表达均呈负相关性(rs=-0.449,-0.765,P=0.000)。随访5~65个月,肿瘤组织TRIM58蛋白阳性表达者5年生存率高于阴性表达者[81.08%(30/37)和50.94%(27/53),P<0.05];且TRIM58基因甲基化者5年生存率低于未甲基化者[53.97%(34/63)和85.19%(23/27),P<0.05]。结论:结直肠癌组织中TRIM58蛋白多呈低表达水平,且与TRIM58基因启动子区域高甲基化状态有关。而且肿瘤组织中TRIM58基因高甲基化状态和蛋白低表达量与患者临床病理特征及预后不良存在一定联系。

Objective: To investigate the aberrant promoter methylation status of tripartite motif 58 (TRIM58) gene and TRIM58 protein level in colorectal cancer and their clinical significance. Methods: The tumor tissues and matched paracancerous tissues of 95 patients with colorectal cancer were selected. Immunohistochemical staining and methylation-specific PCR (MSP) were used to determine the expression of TRIM58 protein and aberrant promoter methylation status of TRIM58 gene. And then the correlation between protein expression and clinicopathological features or prognosis of colorectal cancer patients were investigated.Results: The positive expression rate of TRIM58 protein in tumor tissues was lower than that in adjacent tissues [40.0% (38/95) vs. 75.79% (72/95), P<0.05], which would be associated with tumor size, TNM stage and invasion depth (P<0.05). The aberrant promoter methylation rate of TRIM58 gene in tumor tissues was higher than that in adjacent tissues [69.47% (66/95) vs. 35.79% (34/95), P<0.05], which would be associated with tumor size, tumor differentiation, lymph metastasis, TNM stage and invasion depth (P<0.05). Spearman analysis showed TRIM58 protein was negatively related with TRIM58 gene methylation in tumor tissues and adjacent tissues (rs=-0.449,-0.765, P=0.000). After followed up for 5 to 65 months, 5-year survival rate of patients with positive TRIM58 protein expression was higher than patients with negative TRIM58 protein expression[81.08% (30/37) vs. 50.94% (27/53), P<0.05]. And 5-year survival rate of patients with TRIM58 gene methylation was lower than patients with TRIM58 gene non-methylation [53.97% (34/63) vs. 85.19% (23/27), P<0.05].Conclusion: TRIM58 protein declines frequently, which can be associated with TRIM58 gene promoter methylation. The low TRIM58 protein and TRIM58 gene methylation status is related with clinicopathological features and poor prognosis.

参考文献:

[1] 赫捷,陈万青.2017中国肿瘤登记年报[M].北京:人民卫生出版社,2018.
[2] 张帅,邢影,蔡莉.TRIM家族蛋白在肿瘤中的研究进展[J].现代肿瘤医学,2019,27(11):2025-2028.
[3] LIU L,ZHANG L,WANG J P,et al.Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non-small cell lung cancer xenografts[J].Mol Med Rep,2018,17(1):835-842.
[4] 梁倩,房静远,张洁,等.TRIM55在结直肠癌中的表达及其临床意义[J].胃肠病学,2017,22(6):341-345.
[5] TAO R,LI J,XIN J J,et al.Methylation profile of single hepatocytes derived from hepatitis B virus-related hepatocellular carcinoma[J].PLoS One,2011,6(5):e19862.
[6] TONG G J,ZHANG G Y,LIU J,et al.Comparison of the eighth version of the American Joint Committee on Cancer manual to the seventh version for colorectal cancer:a retrospective review of our data[J].World J Clin Oncol,2018,9(7):148-161.
[7] PATEL A,SIBBET G J,HUANG D T.Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B ubiquitin complex[J].J Biol Chem,2019,294(4):1240-1249.
[8] STEVENS R V,ESPOSITO D,RITTINGER K.Characterisation of class VI TRIM RING domains:linking RING activity to C-terminal domain identity[J].Life Sci Alliance,2019,2(3):e201900295.
[9] 贺敏,陈勇伟,曾康康,等.TRIM14在子宫内膜癌中的表达及其对子宫内膜癌细胞侵袭和转移的影响[J].临床与病理杂志,2019,26(10):2133-2141.
[10] 王文莙,王菲,张弛,等.下调TRIM33基因对人胃癌细胞BGC-823增殖和侵袭的影响[J].解放军医学杂志,2019,44(3):210-214.
[11] EYKING A,FERBER F,KÖHLER S,et al.TRIM58 restrains intestinal mucosal inflammation by negatively regulating TLR2 in myeloid cells[J].J Immunol,2019,203(6):1636-1649.
[12] 张競文,续倩,李国亮,等.癌症发生发展中的表观遗传学研究[J].遗传,2019,41(7):567-581.
[13] 郭玺,董坚.DNA甲基化在结直肠癌中的研究进展[J].西部医学,2019,17(1):157-160.
[14] ZHANG W M,CUI Q C,QU W F,et al.TRIM58/cg26157385 methylation is associated with eight prognostic genes in lung squamous cell carcinoma[J].Oncol Rep,2018,40(1):206-216.
[15] LI Y,GU J,XU F K,et al.Novel methylation-driven genes identified as prognostic indicators for lung squamous cell carcinoma[J].Am J Transl Res,2019,11(4):1997-2012.

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