Objective: To investigate the aberrant promoter methylation status of tripartite motif 58 (TRIM58) gene and TRIM58 protein level in colorectal cancer and their clinical significance. Methods: The tumor tissues and matched paracancerous tissues of 95 patients with colorectal cancer were selected. Immunohistochemical staining and methylation-specific PCR (MSP) were used to determine the expression of TRIM58 protein and aberrant promoter methylation status of TRIM58 gene. And then the correlation between protein expression and clinicopathological features or prognosis of colorectal cancer patients were investigated.Results: The positive expression rate of TRIM58 protein in tumor tissues was lower than that in adjacent tissues [40.0% (38/95) vs. 75.79% (72/95), P<0.05], which would be associated with tumor size, TNM stage and invasion depth (P<0.05). The aberrant promoter methylation rate of TRIM58 gene in tumor tissues was higher than that in adjacent tissues [69.47% (66/95) vs. 35.79% (34/95), P<0.05], which would be associated with tumor size, tumor differentiation, lymph metastasis, TNM stage and invasion depth (P<0.05). Spearman analysis showed TRIM58 protein was negatively related with TRIM58 gene methylation in tumor tissues and adjacent tissues (rs=-0.449,-0.765, P=0.000). After followed up for 5 to 65 months, 5-year survival rate of patients with positive TRIM58 protein expression was higher than patients with negative TRIM58 protein expression[81.08% (30/37) vs. 50.94% (27/53), P<0.05]. And 5-year survival rate of patients with TRIM58 gene methylation was lower than patients with TRIM58 gene non-methylation [53.97% (34/63) vs. 85.19% (23/27), P<0.05].Conclusion: TRIM58 protein declines frequently, which can be associated with TRIM58 gene promoter methylation. The low TRIM58 protein and TRIM58 gene methylation status is related with clinicopathological features and poor prognosis. |
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