外泌体与卵巢相关疾病的研究进展-现代医学

外泌体与卵巢相关疾病的研究进展

单位：上海交通大学医学院附属新华医院妇产科, 上海 200082

分类号：R711.75

出版年·卷·期（页码）：2020·39·第八期（1080-1086）

摘要：

卵巢疾病对女性的身体健康有着严重的危害，很多卵巢相关疾病患者在确诊时已经处于疾病进展期，尤其是卵巢恶性肿瘤。同时对卵巢相关疾病进行治疗也十分困难，并且预后也存在反复复发的情况。外泌体是由多细胞囊泡体和细胞膜融合之后形成的膜性囊泡。外泌体自身可作为信息交流以及细胞间交流的工具，参与细胞间的通讯，把它们的分子运送到特定细胞中。同时外泌体参与多种生理和病理过程，包括癌的发生、免疫调节、蛋白质的转运、血管生成和转移等。本文对外泌体和卵巢相关疾病进行综述，同时分享了外泌体与卵巢相关疾病的研究进展。

Ovarian disease is a serious harm to the health of women. Many patients with ovarian-related diseases are already in the advanced stage of disease, especially malignant ovarian tumors. At the same time, it is very difficult to treat ovarian-related diseases, and the prognosis is recurrent. Exocrine is a membranous vesicle formed by the fusion of multicellular vesicles and cell membrane. Exocrine itself can be used as a tool for information communication and cell-to-cell communication, participating in cell-to-cell communication and transporting their molecules to specific cells. Exocrine is involved in a variety of physiological and pathological processes, including carcinogenesis, immunomodulation, and protein transfer, transport, angiogenesis and metastasis. In this paper, the exocrine and ovarian related diseases were reviewed, and the research progress of exocrine and ovarian related diseases was also shared.

参考文献：

[1] HARDING C,HEUSER J,STAHL P.Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes[J].The Journal of Cell Biology,1983,97(2):329-339.
[2] BUDNIK V,RUIZ-CANADA C,WENDLER F.Extracellular vesicles round off communication in the nervous system[J].Nat Rev Neurosci,2016,17(3):160-172.
[3] SZAJNIK M,DERBIS M,LACH M,et al.Exosomes in plasma of patients with ovarian carcinoma:potential biomarkers of tumor progression and response to therapy[J].Gynecol Obstet (Sunnyvale),2013,Suppl 4:3.
[4] SHARMA S,SALOMON C.Techniques associated with exosome isolation for biomarker development:liquid biopsies for ovarian cancer detection[J].Methods in Molecular Biology,2020,2055:181-199.
[5] JOEL Z N,YI L,PIETER V,et al.Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties[J].Nanomedicine,2015,11(4):879-883.
[6] SHARMA S,ZUNIGA F,RICE G E,et al.Tumor-derived exosomes in ovarian cancer-liquid biopsies for early detection and real-time monitoring of cancer progression[J].Oncotarget,2017,8(61):104687-104703.
[7] CAPPELLESSO R,TINAZZI A,GIURICI T,et al.Programmed cell death 4 and microRNA 21 inverse expression is maintained in cells and exosomes from ovarian serous carcinoma effusions[J].Cancer Cytopathol,2014,122(9):685-693.
[8] SZAJNIK M,CZYSTOWSKA M,SZCZEPANSKI M J,et al.Tumor-derived microvesicles induce,expand and up-regulate biological activities of human regulatory T cells (Treg)[J].PLoS One,2010,5(7):e11469.
[9] LUTGENDORF S K,THAKER P H,AREVALO J M,et al.Biobehavioral modulation of the exosome transcriptome in ovarian carcinoma[J].Cancer,2018,124(3):580-586.
[10] JEPPESEN D K,HVAM M L,PRIMDAHL-BENGTSON B,et al.Comparative analysis of discrete exosome fractions obtained by differential centrifugation[J].J Extracell Vesicles,2014,3:25011.
[11] HU Y,RAO S S,WANG Z X,et al.Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function[J].Theranostics,2018,8(1):169-184.
[12] FANG T,LV H,LV G,et al.Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer[J].Nat Commun,2018,9(1):191.
[13] ZAROVNI N,CORRADO A,GUAZZI P,et al.Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches[J].Methods,2015,87:46-58.
[14] GHOLIZADEH S,SHEHATA D M,ZARGHOONI M,et al.Microfluidic approaches for isolation,detection,and characterization of extracellular vesicles:current status and future directions[J].Biosens Bioelectron,2017,91:588-605.
[15] CECILIA L.Exosomes in diagnostic and therapeutic applications:biomarker,vaccine and RNA interference delivery vehicle[J].Expert Opinion on Biological Therapy,2015,15(1):103-117.
[16] VLASSOV A V,MAGDALENO S,SETTERQUIST R,et al.Exosomes:current knowledge of their composition,biological functions,and diagnostic and therapeutic potentials[J].Biochim Biophys Acta,2012,1820(7):940-948.
[17] SHAO Y,SHEN Y,CHEN T,et al.The functions and clinical applications of tumor-derived exosomes[J].Oncotarget,2016,7(37):60736-60751.
[18] BUYS S S,PARTRIDGE E,BLACK A,et al.Effect of screening on ovarian cancer mortality:the prostate,lung,colorectal and ovarian (PLCO) cancer screening randomized controlled trial[J].JAMA,2011,305(22):2295-2303.
[19] TAYLOR D D,GERCEL-TAYLOR C.MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer[J].Gynecologic Oncology,2008,110:13-21.
[20] WANG W,YIN Y,SHAN X,et al.The value of plasma-based microRNAs as diagnostic biomarkers for ovarian cancer[J].Am J Med Sci,2019,358(4):256-267.
[21] Soltész B,Lukács J,Szilágyi E,et al.Expression of CD24 in plasma,exosome and ovarian tissue samples of serous ovarian cancer patients[J].J Biotechnol,2019,298:16-20.
[22] HUANG X,HAO C,BAO H,et al.Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients[J].Journal of Assisted Reproduction and Genetics,2016,33(1):111-121.
[23] 王洁,王倩,崔趁趁,等.多囊卵巢综合征血清和卵泡液外泌体microRNA的表达谱研究[J].山东医药,2019,59(6):35-38.
[24] XU B,ZHANG Y W,TONG X H,et al.Characterization of microRNA profile in human cumulus granulosa cells:identification of microRNAs that regulate notch signaling and are associated with PCOS[J].Mol Cell Endocrinol,2015,404:26-36.
[25] LUO H,HAN Y,LIU L,et al.Identification of microRNAs in granulosa cells from patients with different levels of ovarian reserve function and the potential regulatory function of miR-23a in granulosa cell apoptosi[J].Gene,2019,686:250-260.
[26] XIAO G Y,CHENG C C,CHANG Y S,et al.Exosomal miR-10a derived from amniotic fluid stem cells preserves ovarian follicles after chemotherapy[J].Sci Rep,2016,6:23120.
[27] HAN S,RHEE W J.Inhibition of apoptosis using exosomes in Chinese hamster ovary cell culture[J].Biotechnol Bioeng,2018,115(5):1331-1339.
[28] PAN C,STEVIC I,MüLLER V,et al.Exosomal microRNAs as tumor markers in epithelial ovarian cancer[J].Mol Oncol,2018,12(11):1935-1948.
[29] NGYH,ROME S,JALABERT A,et al.Endometrial exosomes/microvesicles in the uterine microenvironment:a new paradigm for embryo-endometrial cross talk at implantation[J].PLoS One,2013,8(3):e58502.
[30] 王礼贤,胡月阳,李琰,等.芯片技术分析卵巢子宫内膜异位症外泌体micro RNA表达差异[J].重庆医科大学学报,2019,44(17):885-890.
[31] HARP D,DRISS A,MEHRABI S,et al.Exosomes derived from endometriotic stromal cells have enhanced angiogenic effects in vitro[J].Cell Tissue Res,2016,365(1):187-196.
[32] RASHED M H,KANLIKILICER P,RODRIGUEZAGUAYO C,et al.Exosomal miR-940 maintains SRC-mediated oncogenic activity in cancer cells:a possible role for exosomal disposal of tumor suppressor miRNAs[J].Oncotarget,2017,8(12):20145-20164.
[33] YANG L,WU X,WANG D,et al.Renal carcinoma cell-derived exosomes induce human immortalized line of Jurkat T lymphocyte apoptosis in vitro[J].Urol Int,2013,91(3):363-369.
[34] TODESCHINI P,SALVIATO E,PARACCHINI L,et al.Circulating miRNA landscape identifies miR-1246 as promising diagnostic biomarker in high-grade serous ovarian carcinoma:a validation across two independent cohorts[J].Cancer Lett,2017,388:320-327.
[35] KANLIKILICER P,BAYRAKTAR R,RASHED M,et al.Abstract 1988:exosome-mediated ovarian cancer tumorigenesis mediated by miR1246/Rb/Cav1 axis[J].Cancer Research,2017,77(13):1988.
[36] PINK R C,SAMUEL P,MASSA D,et al.The passenger strand,miR-21-3p,plays a role in mediating cisplatin resistance in ovarian cancer cells[J].Gynecol Oncol,2015,137(1):143-151.
[37] ZHANG F F,ZHU Y F,ZHAO Q N,et al.Microvesicles mediate transfer of P-glycoprotein to paclitaxel-sensitive A2780 human ovarian cancer cells,conferring paclitaxel-resistance[J].Eur J Pharmacol,2014,738:83-90.
[38] CAO Y L,ZHUANG T,XING B H,et al.Exosomal DNMT1 mediates cisplatin resistance in ovarian cancer[J].Cell Biochem Funct,2017,35(6):296-303.
[39] MARLEAU A M,CHEN C S,JOYCE J A,et al.Exosome removal as a therapeutic adjuvant in cancer[J].J Transl Med,2012,10:134.
[40] AZMI A S,BAO B,SARKAR F H.Exosomes in cancer development,metastasis and drug resistance:a comprehensive review[J].Cancer Metastasis Rev,2013,32(3-4):623-642.
[41] PASCUCCI L,COCCE V,BONOMI A,et al.Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth:a new approach for drug delivery[J].J Control Release,2014,192:262-270.
[42] TIAN Y,LI S,SONG J,et al.A doxorubicin delivery platform using engineered natural membrane vesicle exosomes for targeted tumor therapy[J].Biomaterials,2014,35(7):2383-2390.
[43] OGATA-KAWATA H,IZUMIYA M,KURIOKA D,et al.Circulating exosomal microRNAs as biomarkers of colon cancer[J].PLoS One,2014,9(4):e92921.
[44] LIU J,SUN H,WANG X,et al.Increased exosomal microRNA-21 and microRNA-146a levels in the cervicovaginal lavage specimens of patients with cervical cancer[J].Int J Mol Sci,2014,15(1):758-773.
[45] GILAD S,MEIRI E,YOGEV Y,et al.Serum microRNAs are promising novel biomarkers[J].PLoS One,2008,3(9):e3148.
[46] LV L L,CAO Y H,NI H F,et al.MicroRNA-29c in urinary exosome/microvesicle as a biomarker of renal fibrosis[J].Am J Physiol Renal Physiol,2013,305(8):F1220-1227.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录