Objective: To investigate the value of combined use of procalcitonin (PCT),C-reactive protein (CRP) and blood glucose (GLU) in the early diagnosis of intracranial infection after craniocerebral operation.Methods: From January 2016 to December 2018, 39 patients with intracranial infection after cranial surgery treated at the Department of Neurosurgery in Cangzhou People's Hospital were used as experimental group, and 62 cases without infection were used as control group. Serum PCT, CRP and GLU levels were measured in the two groups, and the diagnostic value of the ROC curve analysis indicators was analyzed.Results: One day before surgery, there was no significant difference in serum levels of GLU, PCT and CRP between the experimental group and the control group. Compared with the day before surgery, the serum levels of GLU, PCT and CRP in the experimental group were significantly higher than those two days after surgery (P<0.01). There was no significant change in the control group. GLU was positively correlated with PCT (r=0.708, P<0.01) and CRP (r=0.390 9, P<0.01). The areas under the GLU, PCT, and CRP curves were 0.770 5, 0.894 5, and 0.846 7, respectively (P<0.01). The best detection critical points of GLU, PCT and CRP were at 5.28 mmol·L^-1, 0.27 ng·ml^-1 and 15.71 mg·L^-1. The area under the ROC curve for the combined diagnosis of GLU, PCT and CRP was 0.935 5(P<0.01), which was higher than that of the single index. Conclusion: The combination of GLU, PCT and CRP can efficiently diagnose intracranial infections after craniocerebral surgery.