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甘草次酸对甲状腺癌SW579细胞放射敏感性的影响
作者:田甜  莫淑婵  吴钟彪  江聪 
单位:海南省海口市中医医院 外科, 海南 海口 570216
关键词:甘草次酸 甲状腺癌 放疗敏感性 自噬 
分类号:R965.2
出版年·卷·期(页码):2020·48·第十一期(1381-1384)
摘要:

目的:研究甘草次酸(glycyrrhetinic acid,GA)对甲状腺癌SW579细胞的放射增敏作用,以及其作用的可能机制。方法:四氮噻唑蓝(MTT)细胞毒实验检测细胞生长抑制率,流式细胞术检测细胞凋亡,蛋白质免疫印迹法(Western bloting)检测自噬蛋白LC3-Ⅰ和LC3Ⅱ表达。结果:MTT细胞毒实验结果显示,5、10、20、40、80 μmol·L-1 GA处理甲状腺癌SW579细胞,生长抑制率分别为(4.64±0.83)%、(18.37±3.11)%、(33.54±6.32)%、(48.24±7.16)%和(82.32±12.23)%,各组间差异具有统计学意义(P<0.01)。5 μmol·L-1 GA联合2、4、8、16、32 Gy射线处理细胞的抑制率显著高于单独的2、4、8、16、32 Gy射线处理细胞的抑制率(P<0.01)。流式细胞术凋亡检测结果显示,5 μmol·L-1 GA+8 Gy射线组的SW579细胞凋亡率显著高于8 Gy射线组(P<0.001)。Western bloting结果显示,8 Gy射线组LC3Ⅱ/LC3-Ⅰ值显著高于对照组(P<0.01),5 μmol·L-1 GA+8 Gy射线组LC3Ⅱ/LC3-Ⅰ值显著低于8 Gy射线组(P<0.01)。结论:GA可以抑制放射线激发的甲状腺癌细胞自噬增强,显著增加甲状腺癌细胞对放射线的敏感性。

Objective: To investigate radiosensitizing effect of glycyrrhetinic acid on SW579 cells and its possible mechanism. Methods: MTT assay was used to detect the inhibition rate of cell growth. Apoptosis was detected by flow cytometry and protein expression of LC3-Ⅰ and LC3Ⅱ was detected by Western bloting. Results: After treated with 5, 10, 20, 40, and 80 μmol·L-1 GA, the growth inhibition rates of SW579 cells were (4.64±0.83)%, (18.37±3.11)%, (33.54±6.32)%, (48.24±7.16)% and (82.32±12.23)%, respectively, with significant differences between the groups(P<0.001). The growth inhibition rate of 5 μmol·L-1 GA combined with 2, 4, 8, 16 and 32 Gy radiation treatment was significantly higher than that of 2, 4, 8, 16 and 32 Gy radiation treatment alone(P<0.01). The apoptosis rate of SW579 cells in 5 μmol·L-1 GA+8 Gy radiation treatment group was significantly higher than that in 8 Gy radiation treatment group (P<0.01). The ratio of LC3Ⅱ/LC3-Ⅰ in 8 Gy radiation treatment group was significantly higher than that in control group (P<0.01). The ratio of LC3Ⅱ/LC3-Ⅰ in 5 μmol·L-1 GA+8 Gy radiation treatment group was significantly lower than that in 8 Gy radiation treatment group (P<0.01). Conclusion: Glycyrrhetinic acid can inhibit autophagy induced by radiation and increase the sensitivity of thyroid cancer cells to radiation.

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