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Ki-67抗原和P53蛋白在胃黏液腺癌组织中的表达及预后分析
作者:王颖  刘申香  童建东 
单位:扬州大学附属医院 肿瘤科, 江苏 扬州 225001
关键词:胃黏液腺癌 Ki-67抗原 P53蛋白 免疫组织化学 预后 
分类号:R735.2
出版年·卷·期(页码):2020·48·第十一期(1413-1419)
摘要:

目的:探究Ki-67抗原和P53蛋白在胃黏液腺癌组织中的表达及预后意义。方法:回顾性分析扬州大学附属医院2010年至2014年41例胃黏液腺癌病例,采用免疫组化方法检测41例胃黏液腺癌组织中Ki-67抗原和P53蛋白的表达,并分析其表达水平与临床病理特征及预后的关系。结果:P53蛋白和Ki-67抗原在胃黏液腺癌组织中的阳性表达率分别为58.50%和46.30%。Ki-67抗原表达水平与胃黏液腺癌浸润深度和淋巴结转移密切相关(P<0.05)。P53蛋白表达水平与胃黏液腺癌浸润深度、淋巴结转移及TNM分期密切相关(P<0.05)。生存分析表明P53蛋白阳性表达及Ki-67抗原阳性表达均与较差的术后3年生存时间显著相关(P53蛋白:P=0.025;Ki-67抗原阳性:P=0.023)。此外,P53蛋白及Ki-67抗原均阳性表达的患者较两者均阴性表达的患者有着更短的术后3年生存时间(P=0.002)。单因素和多因素分析表明P53蛋白表达水平、Ki-67抗原表达水平、患者年龄、肿瘤分化程度均是影响胃黏液腺癌术后生存时间的独立预后因素(P<0.05)。结论:P53蛋白和Ki-67抗原表达水平与胃黏液腺癌病程进展及预后显著相关,联合检测两者表达水平有望成为该疾病临床预后精准分型的新策略。

Objective: To investigate the expression and prognostic significance of Ki-67 and P53 in gastric mucinous adenocarcinoma. Methods: A retrospective cohort of 41 gastric mucinous adenocarcinoma cases from 2010 to 2014 were analyzed inAffiliated Hospital of Yangzhou University. The immunohistochemistry was used to detect the expression of Ki-67 and P53 in 41 cases of gastric mucinous adenocarcinoma tissues. The correlations of their expression with clinicopathological features and patient prognosis were analyzed. Results: The positive expression of P53 and Ki-67 were detected in 58.50%and 46.30%of mucinous adenocarcinoma tissues respectively. Theexpression of Ki-67 was significantly correlated with invasion and lymph node metastasis, whilethe expression of P53 was significantly correlated with invasion, the lymph node metastasis and TNM staging in gastric mucinous adenocarcinoma (P<0.05). The survival analysis revealed the positive expression of P53 and Ki-67 was both significantly correlated with shorter three-year survival after surgery (P=0.025 for P53 and P=0.023 for Ki-67). Moreover, patients with simultaneous positive P53 and Ki-67 expression had a significantly worse three-year survival than those with simultaneous negative P53 and Ki-67 expression (P=0.002). The univariate and multivariate analysis showed P53 expression, Ki-67 expression, age and tumor differentiation were independent prognostic factors for postoperative survival of gastric mucinous carcinoma (P<0.05). Conclusion: Both the expression of P53 and Ki-67 were significantly correlated with the development and prognosis of gastric mucinous adenocarcinoma. Combined detection for their expression may be a novel strategy for precise prognostic stratification in patients with gastric mucinous adenocarcinoma.

参考文献:

[1] 陈万青, 李贺,孙可欣,等. 2014年中国恶性肿瘤发病和死亡分析[J].中华肿瘤杂志, 2018, 40(1):5-12.
[2] 秦新裕,刘凤林.努力提高胃癌的术后5年生存率[J].J Surg Concepts Pract, 2008,13(1):4-6.
[3] KUNISAKI C, AKIYAMA H, NOMURA M,et al. Clinicopathologiccharacteristics and surgical outcomes of mucinous gastriccarcinoma[J]. Ann Surg Oncol, 2006,13(6):836-842.
[4] WOO L S, KIM D Y, KIM Y J, et al. Clinicopathologic features of mucinous gastric carcinoma[J].Dig Surg, 2002,19(4):286-290.
[5] WEN L F, CHEN W W, SU S L, et al.Mucin-producing gastric caner:clinicopathological difference between signet ring cell carcinoma and mucinous carcinoma[J].Hepato-Gastroenterology, 2009,56:1227-1231.
[6] NICOLETTI M J, MYERS T G, FOJO T, et al.Wild-type P53 marginally induces endogenous MDR-1 mRNA without causing a measurable drug resistance in human cancer cells[J].Int J Onol, 2001,18(2):375-381.
[7] 方刚, XUE C Y,韩晓冬.小分子干扰RNA片段逆转KBv200细胞多药耐药的实验研究[J].医学研究生学报, 2006,19(2):104-106.
[8] VOUSDEN K H, LU X.Live or let die:the cell's response to P53[J].Nat Rev Cancer, 2002,2(8):594-604.
[9] 林丹丹,张一桥.胃癌组织中P53与Ki-67蛋白的表达变化及意义[J].山东医药2015,55(45):48-49.
[10] 李刚强,周海亚,朱瑞.Bub1和P53在胃癌中的表达及意义[J].现代肿瘤医学, 2001,7(19):1391-1394.
[11] 崔轶霞,王海军,惠起源.胃黏膜不同病变组织中突变型P53蛋白表达的研究[J].延安大学学报(医学科学版), 2008,6(3):3-4.
[12] KARIAM S, ALI A.Correlation of P53 over-exprcssion and ahemtion in P53 gone detected by polymerase chain reaction-single strand conformation polymorphism in adenocareinoma of gastric canoer patients from India[J].World J Gastroenterol,2009,15(11):138l-1387.
[13] 房殿春,罗元辉,鲁荣,等.胃癌组织P53基因突变、蛋白产物的表达及病人预后关系[J].第三军医大学报, 1994,16(6):418.
[14] VICTORZON M, NORDLING S, HAGLUND C,et al.Expression of P53 protein as aprognostic factor in patients with gastric cancer[J].Eur J Cancer, 1996,32A(2):215-220.
[15] CZYZEWSKA J, GUZINSKA-USTYMOWICZ K, LEBELT A,et al. Evaluation of p roliferating markers Ki-67, PCNA in gastric cancers[J].Rocz Akad Med Bialymst, 2004,49(suppl.1):64-66.
[16] TZANAKIS N E, PEROS G, KARAKITSOS P, et al. prognostic signifi cance of P53 and Ki67 proteins expression in Greek gastric cancer patients[J].Acta Chir Belg, 2009,109(5):606-611.
[17] 易伟,胡志前,龚振斌. Ki67抗原和P53蛋白在胃癌中的表达及其临床意义[J].中国普通外科杂志,2006,15(9):664-667.

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