目的： 探讨LncRNA HCP5调节has-miR-600/HMGB1轴对非小细胞肺癌（NSCLC） A549细胞的影响机制。方法： TCGA数据库配对NSCLC病人肿瘤组织和癌旁组织的基因表达；GEO数据库筛选差异表达的miRNA；质粒构建和转染A549细胞过表达has-miR-600或过表达LncRNA HCP5，噻唑蓝（MTT）法检测细胞增殖，细胞迁移实验检测细胞侵袭能力。结果： 肿瘤组织中高迁移率族蛋白B1（HMGB1）表达量为（13.6±1.38），显著高于癌旁组织的（12.8±1.27），差异有统计学意义（t=2.444，P=0.040）；A549细胞中过表达has-miR-600降低HMGB1的表达（t=2.859，P=0.021），降低细胞的增殖和侵袭能力（t=2.571，P=0.033；t=2.418，P=0.042）；A549细胞中过表达LncRNA HCP5增加HMGB1蛋白水平（t=2.446，P=0.040），细胞侵袭能力增强（t=2.389，P=0.044）；而has-miR-600与LncRNA HCP5共转染则减弱LncRNA HCP5过表达的影响。结论： LncRNA HCP5调控has-miR-600/HMGB1轴影响非小细胞肺癌A549细胞的增殖和侵袭，可为NSCLC的治疗提供一个潜在的新靶点。

[1] RANA R H,ALAM F,ALAM K,et al.Gender-specific differences in care-seeking behaviour among lung cancer patients:a systematic review[J].J Cancer Res Clin Oncol,2020,146(5):1169-1196.
[2] PATEL A,BATRA U,PRASAD K T,et al.Real world experience of treatment and outcome in ALK-rearranged metastatic nonsmall cell lung cancer:a multicenter study from India[J].Curr Probl Cancer,2020,44(3):100571.
[3] WU X J,CHEN Y Y,GONG C C,et al.The role of high-mobility group protein box 1 in lung cancer[J].J Cell Biochem,2018,119(8):6354-6365.
[4] ZHU J,LUO J,LI Y,et al.HMGB1 induces human non-small cell lung cancer cell motility by activating integrin αvβ3/FAK through TLR4/NF-κB signaling pathway[J].Biochem Biophys Res Commun,2016,480(4):522-527.
[5] CHI Y,WANG D, WANG J,et al.Long non-coding RNA in the pathogenesis of cancers[J].Cells,2019,8(9):1015.
[6] GHAFOURI-FARD S,SHOOREI H,BRANICKI W,et al.Non-coding RNA profile in lung cancer[J].Exp Mol Pathol,2020,114:104411.
[7] WU H,ZHOU C.Long non-coding RNA UCA1 promotes lung cancer cell proliferation and migration via microRNA-193a/HMGB1 axis[J].Biochem Biophys Res Commun,2018,496(2):738-745.
[8] 兰飞,赵鹏,刘冬,等.LncRNA DANCR调控非小细胞肺癌细胞的凋亡和上皮间质转化[J].现代医学,2020,48(1):75-82.
[9] LI C,LEI Z,PENG B,et al.LncRNA HCP5 stimulates the proliferation of non-small cell lung cancer cells by up-regulating survivin through the down-regulation of miR-320[J].Cancer Manag Res,2020,12:1129-1134.
[10] CHEN J,ZHAO D,MENG Q.Knockdown of HCP5 exerts tumor-suppressive functions by up-regulating tumor suppressor miR-128-3p in anaplastic thyroid cancer[J].Biomed Pharmacother,2019,116:108966.
[11] XIA J,LI D,ZHU X,et al.Upregulated miR-665 expression independently predicts poor prognosis of lung cancer and facilitates tumor cell proliferation,migration and invasion[J].Oncol Lett,2020,19(5):3578-3586.
[12] XU F,ZHANG H,SU Y,et al.Up-regulation of microRNA-183-3p is a potent prognostic marker for lung adenocarcinoma of female non-smokers[J].Clin Transl Oncol,2014,16(11):980-985.
[13] YANG Z D,KANG H.Exploring prognostic potential of long noncoding RNAs in colorectal cancer based on a competing endogenous RNA network[J].World J Gastroenterol, 2020,26(12):1298-1316.
[14] 熊英,程佳.血清长链非编码RNA PVT1联合CA125与CEA在子宫内膜癌中的临床价值研究[J].现代医学,2019,47(8):987-992.
[15] CHENG K J,ALSHAWSH M A,MEJIAMOHAMED E H,et al.HMGB1:an overview of its versatile roles in the pathogenesis of colorectal cancer[J].Cell Oncol,2020,43(2):177-193.