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miR-122-5p靶向FGFRL1通过PI3K/AKT信号通路抑制胶质瘤细胞的增殖、迁移和侵袭
作者:魏俊  杨珉 
单位:孝感市中心医院 神经外科, 湖北 孝感 342000
关键词:胶质瘤 微小RNA-122-5p 成纤维细胞生长因子受体样1 增殖 迁移 侵袭 
分类号:R739.41
出版年·卷·期(页码):2021·49·第一期(5-12)
摘要:

目的: 探讨微小RNA-122-5p(miR-122-5p)对胶质瘤细胞增殖、迁移及侵袭能力的影响及其可能作用机制。方法: 采用qRT-PCR和Western blotting检测HEB细胞及胶质瘤SHG44、U87细胞中miR-122-5p、成纤维细胞生长因子受体样1(FGFRL1)的表达;MTT、Transwell小室实验分别检测miR-122-5p过表达与敲低FGFRL1表达对SHG44细胞增殖、迁移及侵袭能力的影响;双荧光素酶报告基因检测miR-122-5p与FGFRL1基因的靶向关系。FGFRL1过表达验证 miR-122-5p对SHG44细胞增殖、迁移及侵袭的作用。采用Western blotting检测MMP2、MMP9及PI3K/AKT信号通路蛋白的表达水平。结果:胶质瘤细胞中miR-122-5p的表达下调,FGFRL1的表达上调;miR-122-5p过表达或敲低FGFRL1可显著抑制胶质瘤细胞增殖、迁移及侵袭能力,抑制MMP2、MMP9、p-PI3Kp85、p-AKT表达。双荧光素酶报告实验证实FGFRL1基因是miR-122-5p的靶基因。FGFRL1过表达可逆转miR-122-5p过表达对胶质瘤细胞增殖、迁移及侵袭的抑制作用。结论: miR-122-5p可负向调控FGFRL1表达而抑制胶质瘤细胞增殖、迁移及侵袭,其可能通过调控PI3K/AKT信号通路而发挥作用。

Objective: To investigate the effects of microRNA-122-5p (miR-122-5p) on the proliferation, migration and invasion of glioma cells and its possible mechanism.Methods: qRT-PCR and Western blotting were used to detect the expression of miR-122-5p and fibroblast growth factor receptor-like 1(FGFRL1) in HEB cells and glioma SHG44 and U87 cells. MTT assay and Transwell chamber assay were used to detect the effects of miR-122-5p overexpression and knockdown of FGFRL1 on proliferation, migration and invasion of SHG44 cells. The dual luciferase reporter gene was used to detected the target relationship between miR-122-5p and the FGFRL1 gene. Overexpression of FGFRL1 verified the effect of miR-122-5p on proliferation, migration and invasion of SHG44 cells. The expression levels of MMP2, MMP9 and PI3K/AKT signaling pathway proteins were detected by Western blotting. Results: The expression level of miR-122-5p was significantly decreased in glioma cells, while the expression level of FGFRL1 was significantly increased. Overexpression miR-122-5p or knockdownFGFRL1significantly inhibited the proliferation, migration and invasion of glioma cells and inhibited the expression of MMP2, MMP9, p-PI3Kp85 and p-AKT. The dual luciferase reporter assay confirmed that the FGFRL1 gene was a target gene for miR-122-5p. Overexpression of FGFRL1 reversed the inhibitory effect of miR-122-5p overexpression on proliferation, migration and invasion of glioma cells.Conclusion: miR-122-5p can negatively regulate FGFRL1 expression and inhibit glioma cell proliferation, migration and invasion, which may play a role in regulating PI3K/AKT signaling pathway.

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