Objective: To detect the expression of microRNA-762(miR-762) in oral squamous cell carcinoma, and to explore its relationship with cell proliferation and metastasis. Methods: 62 patients with oral squamous cell carcinoma were collected. Tumor tissues were selected as observation group, normal oral mucosa tissues(>2 cm from the edge of the tumors) were selected as control group. miR-762 was detected by real-time fluorescence quantitative PCR in the two groups. Ki67, MMP-2 and MMP-9 were detected by immunohistochemical method in observation group. Ki67, MMP-2 and MMP-9 were detected by Western blotting method in observation group. Results: The expression of miR-762 was significantly higher in observation group than that in control group. The expression of miR-762 was statistically significant in different vascular recidivism, lymph node metastasis and different TNM stages, but was not statistically significant in gender, age and differentiation in the observation group. The expression of miR-762 was closely related to survival time. Positive correlation was found between miR-762 and maximum diameter, miR-762 and Ki67 proliferation index, miR-762 and MMP-2 expression, miR-762 and MMP-9 expression. Conclusion: The increased expression of miR-762 is related to the formation and progression in oral squamous cell carcinoma. Expression of miR-762 can promote cell proliferation. The metastasis regulation of miR-762 may be mediated by MMP-2 and MM-9. Expression of miR-762 may be related to prognosis.
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