目的：探讨let-7c在非小细胞肺癌细胞生长和自噬中的作用。方法：采用测序方法找寻肺癌组织中表达差异显著的miRNAs，通过RT-qPCR验证测序结果，采用细胞转染技术对非小细胞肺癌进行过表达构建，随后使用RT-qPCR检测转染效率，并经CCK8实验、Western blot实验及免疫荧光实验验证let-7c对肺癌细胞生长和自噬调控因子的影响。结果：let-7c在肺癌组织以及肺癌细胞中的表达降低，过表达let-7c不仅抑制非小细胞肺癌细胞增殖，同时还抑制非小细胞肺癌细胞自噬的发生。结论：let-7c在非小细胞肺癌中发挥抑癌基因作用，有望成为潜在的治疗靶点。

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