SHCBP1在非小细胞肺癌发生发展中的作用-现代医学

SHCBP1在非小细胞肺癌发生发展中的作用

单位：赣南医学院第一附属医院肿瘤科, 江西赣州 341000

分类号：R734

出版年·卷·期（页码）：2021·49·第六期（660-665）

摘要：

目的：探讨纺锤体相关蛋白1（SHCBP1）在非小细胞肺癌（NSCLC）发生发展中的作用。方法：选取2018年1月到2020年3月在赣南医学院第一附属医院治疗的NSCLC患者62例，通过RT-PCR、Werstern blotting和免疫组化法检测SHCBP1基因在NSCLC中的表达并检测其蛋白的表达与定位；利用试剂盒构建SHCBP1质粒并合成出具有特异性的siRNA及shRNA，将之转染至NSCLC细胞株，利用RT-PCR和Western blotting对mRNA及其蛋白表达量进行检测，通过CCK-8实验以及克隆形成检测SHCBP1对NSCLC增殖的影响；利用划痕实验检测过表达及SHCBP1对NSCLC细胞迁移能力的影响。结果：在所检测的样本中，NSCLC组织中SHCBP1的表达相比较非癌组织更高（P<0.01）；SHCBP1在NSCLC组织中主要表达于细胞的细胞质中。过表达研究发现SHCBP1能对NSCLC的增殖及迁移起到促进作用，对SHCBP1进行干扰能够抑制增殖和迁移。结论：SHCBP1在NSCLC中能够促进细胞增殖和迁移，对其发生过程有促进作用。

Objective: To explore therole of SHCBP1 in the development of non-small cell lung cancer(NSCLC). Methods: Select 62 patients with NSCLCtreated in the firstaffiliated hospital of Gannan medical university from January 2018 to March 2020, and detected the expression of SHCBP1 gene in NSCLC by RT-PCR, Werstern blotting and immunohistochemistry and detected its protein expression and localization of SHCBP1; used the kit to construct SHCBP1 plasmid and synthesized specific siRNA and shRNA to transfect it into non-small cell lung cancer cell lines, used RT-PCR and Western blotting to detect mRNA and its protein expression, through CCK8 and clone formation to detect the effect of SHCBP1 on the proliferation of NSCLC; used the scratch test to detect the effect of overexpression and SHCBP1 on the migration ability of NSCLC cells. Results: Among the samples tested, the expression of SHCBP1 in NSCLC tissue was higher than that in non-cancer tissue (P<0.01); the expression of SHCBP1 in NSCLC was mainly found in the cytoplasm of cells. After overexpression studies, it was found that SHCBP1 can promote the proliferation and migration of NSCLC, and interference with SHCBP1 can inhibit proliferation and migration. Conclusion: SHCBP1 can promote cell proliferation and migration in non-small cell lung cancer, and has a role in promoting its occurrence.

参考文献：

[1] 黄佳茹,赵云峰,丁薇,等.非编码RNA HOTAIR和MALAT1在非小细胞肺癌患者血清及支气管肺泡灌洗液中表达水平的临床意义[J]. 东南大学学报(医学版), 2019, 38(4):682-686.
[2] ZHANG G Y,MA Z J,WANG L,et al,The role of shcbp1 in signaling and disease[J]. Curr Cancer Drug Targets, 2019,19(11):854-862.
[3] 马雅莉,叶飞,袁啸,等.非小细胞肺癌患者CD8+CD56+NKT细胞亚群的特性研究[J]. 东南大学学报(医学版),2019,38(6):1012-1018.
[4] DONG Y D,YUAN Y L,YU H B,et al.SHCBP1 is a novel target and exhibits tumor-promoting effects in gastric cancer[J]. Oncol Rep, 2018,41(3):1649-1657.
[5] 陶汉川,王珵,赵晶晶,等.SHCBP1基因在结肠癌组织中表达及对结肠癌细胞增殖迁移的影响[J]. 现代肿瘤医学,2019(13):2244-2249.
[6] CHEN X,CHENG Z R,ZHANG S J,et al. Combining genome wide association studies and differential gene expression data analyses identifies candidate genes affecting mastitis caused by two different pathogens in the dairy cow[J]. OJAS, 2015, 5:358-393.
[7] PENG C L,ZHAO H,SONG Y, et al. SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis[J]. J Exp Clin Cancer Res,2017,36(1):141-145.
[8] 熊磊,刘小龙,徐杨,等.PD-L1在非小细胞肺癌中的表达及其临床意义[J]. 现代医学, 2020,48(9):75-79.
[9] 黄健,王于理,代平,等.胸水、血液EGFR基因突变检测在非小细胞肺癌治疗中的应用价值[J]. 山东医药,2018(6):61-63.
[10] 许浩然,何世杰,孙亚东,等.非小细胞肺癌组织PTEN、p-Akt及p-mTOR的表达及意义[J]. 广东医学, 2018(5):710-716.
[11] 王莉新,吴文斌,朱诗国,等.非小细胞肺癌免疫治疗的策略与展望[J]. 现代免疫学,2018,38(3):247-251.
[12] COOPER W A,TRAN T,VILAIN R E, et al. PD-L1 expression is a favorable prognostic factor in early stage non-small cell carcinoma[J]. Lung Cancer,2015, 89(2):181-188.
[13] SNIDER J T,BATT K,WU Y, et al. The option value of innovative treatments for non-small cell lung cancer and renal cell carcinoma[J]. Am J Manag Care,2017,23(10):e340-e346.
[14] 卢尚辉,张小利,许信杰,等.奥希替尼在非小细胞肺癌中的耐药机制及治疗策略[J]. 肿瘤,2019(12):1031-1036.
[15] 邢若,王鹏,刘玉豪.贝伐珠单抗辅助常规化疗治疗非小细胞肺癌的临床研究[J]. 中国临床药理学杂志,2019,35(1):19-21.
[16] CHENG L,ZHAN B,LUO P,et al. MiRNA-375 regulates the cell survival and apoptosis of human non-small cell carcinoma by targeting HER2[J]. Mol Med Rep, 2017, 15(3):1387-1392.
[17] 王洪阁.非小细胞肺癌患者癌胚抗原和细胞角蛋白与表皮生长因子基因突变的关系及靶向治疗的预测价值[J]. 中国老年学杂志,2019,39(4):46-48.
[18] ZHANG H,ZHANG CF,CHEN R,et al.Zinc finger RNA-binding protein promotes non-small-cell carcinoma growth and tumor metastasis by targeting the Notch signaling pathway[J]. Am J Cancer Res,2017,7(9):1804-1819.

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