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CD64、PCT、CRP在小儿脓毒血症早期诊断的效能比较
作者:陈雷1  方代华2 
单位:1. 江苏省邳州市人民医院 检验科, 江苏 徐州 221300;
2. 徐州医科大学附属徐州儿童医院 输血科, 江苏 徐州 221006
关键词:中性粒细胞CD64 降钙素 C反应蛋白 脓毒血症 
分类号:R722.13
出版年·卷·期(页码):2021·49·第八期(852-855)
摘要:

目的:探讨中性粒细胞CD64、降钙素(Procalcitonin,PCT))、C反应蛋白(C-reactive protein,CRP)在小儿脓毒血症早期临床诊断中的价值。方法:回顾性分析我院2019年9月至2020年9月收治的确诊小儿脓毒血症患者80例,设为脓毒血症组;招募正常健康小儿89名,设为对照组。流式细胞术检测CD64,化学发光法检测血清PCT,免疫荧光法检测血清CRP。结果:脓毒血症组患儿年龄及性别分布与对照组相比无统计学差异(P>0.05);脓毒血症组患儿CD64指数、PCT水平、CRP水平均高于对照组(P<0.05);CD64、PCT、CRP两两之间均存在相关性;受试者工作特征(receiver operating characteristic,ROC)曲线显示,CD64诊断小儿脓毒血症的灵敏度及特异度高于PCT和CRP,曲线下面积(area under the curve,AUC)值也明显高于PCT及CRP (Z=5.27,P<0.001;Z=5.64,P<0.001)。结论:外周血CD64对小儿脓毒血症具有早期诊断价值,明显优于PCT及CRP。

Objective: To explore neutrophil CD64, Procalcitonin(PCT), and C-reactive protein(CRP) for the early clinical diagnosis of sepsis in children.Methods: A retrospective analysis was conducted, 80 confirmed pediatric sepsis children were allocated into sepsis group from September 2019 to September 2020, 89 healthy children were recruited as the control group. CD64 was detected by Flow cytometry, serum PCT was detected by chemiluminescence, and immunofluorescence method was used to detect serum CRP.Results: The age and gender distribution was not statistically different between the two groups(P>0.05); CD64, PCT, and CRP of children in the sepsis group were higher than those of the control group(P<0.05); CD64, PCT, and CRP were significantly positively correlated with each other; receiver operating characteristic(ROC) curve showed that the sensitivity and specificity of CD64 were better than PCT and CRP for early the diagnosis of neonatal sepsis in children. The area under the curve(AUC) value was also significantly higher than that of PCT and CRP(Z=5.27, P<0.001; Z=5.64, P<0.001). Conclusion: CD64 is a better diagnostic biomarker for early diagnosis of neonatal sepsis in children as compared to PCT and CRP.

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