基于生物信息学分析WDR43在肝细胞肝癌中的表达及临床价值-现代医学

基于生物信息学分析WDR43在肝细胞肝癌中的表达及临床价值

单位：1. 安徽医科大学研究生学院, 安徽合肥 230000;
2. 安徽医科大学第一附属医院普外科, 安徽合肥 230000

分类号：R735.7

出版年·卷·期（页码）：2021·49·第八期（870-876）

摘要：

目的：通过数据挖掘的方法探究WD重复蛋白43（WDR43）在肝细胞肝癌（HCC）中的表达水平，分析其与HCC预后及肿瘤免疫浸润的相关性。方法：通过检索肿瘤免疫评估（TIMER）数据库和GEPIA数据库获得WDR43在HCC组织和正常肝组织中的差异性表达，检索WDR43在Kaplan-Meier Plotter数据库的HCC预后结果，然后通过GEPIA数据库进行验证；检索Timer数据库获得WDR43与HCC组织免疫浸润水平及肿瘤相关巨噬细胞表面标志物的相关性。结果：WDR43在HCC中的表达较正常肝组织高，且随着HCC临床分期的升高，WDR43的表达存在上调趋势；WDR43高表达与HCC预后不良呈正相关（[总生存率（OS）：HR=1.7，P=0.002 9；无病生存率（DFS）：HR=1.4，P=0.023]；WDR43表达促进HCC免疫细胞（B细胞、CD4⁺T细胞、CD8⁺T细胞、中性粒细胞、巨噬细胞和树突状细胞）浸润（P<0.01）；HCC中WDR43表达水平与多数免疫细胞标志物正相关且存在明显差异（P<0.001）。结论：通过多个数据库研究发现WDR43在HCC中高表达，并且与HCC的不良预后以及肿瘤相关免疫细胞浸润有关。

Objective: To explore the expression level of WD repeat protein 43(WDR43) in hepatocellular carcinoma(HCC) by data mining, and analyze its correlation with prognosis and tumor immune infiltration. Methods: The differential expression of WDR43 in HCC tissues and normal liver tissues was obtained by searching tumor immune estimation resource(TIMER) database and GEPIA database.The prognosis of WDR43 in Kaplan-Meier Plotter database was retrieved, and then verified by GEPIA database.The correlation between WDR43 and the level of HCC tissue immune infiltration and tumor-related macrophage surface markers was searched in the TIMER database. Results: The expression of WDR43 in HCC was higher than that in normal liver tissue, and with the clinical stage of HCCincreased, the expression of WDR43 had an up-regulating trend; high expression of WDR43 was positively correlated with poor prognosis of HCC(OS:HR=1.7, P=0.002 9; DFS:HR=1.4, P=0.023); WDR43 expression promoted the infiltration of HCC immune cells(B cells, CD4⁺T cells, CD8⁺T cells, neutrophils, macrophages and dendritic cells)(P<0.01); WDR43 expression level in HCC was positively correlated with most immune cell markers and there were significant differences(P<0.001). Conclusion: Through multiple database studies, it is found that WDR43 is highly expressed in HCC and is related to the poor prognosis of HCC and tumor-related immune cell infiltration.

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