Objective: To study the effects of miR-29a on cell proliferation, migration, invasion and phosphatase and tensin homologue-deleted chromosome ten gene/protein kinase B/glycogen synthase kinase-3β(PTEN/AKT/GSK-3β) pathway of glioblastoma multiforme cells(GBM).Methods: GBM cell line TG-905 was cultured in vitro and divided into blank control group(NG group), negative control group(mimics-NC group), miR-29a overexpression group(miR-29a-mimics group), and PTEN inhibitor group(miR-29a-mimics+Bpv group). CCK-8 method and plate cloning experiment were used to detect cell proliferation in each group; Scratch test and Transwell test were used to detect cell migration and invasion ability of each group respectively; Western blotting was used to detect cell proliferating cell nuclear antigen(PCNA), migration and invasion-related proteins such as matrix metalloproteinase 9(MMP-9), E-cadherin, Vimentin, N-cadherin,as well as pathway related protein PTEN, p-PI3K, PI3K, p-AKT, AKT, GSK-3β, β-catenin.Results: The cells were successfully transfected; compared with the NG group and the mimics-NC group, the TG-905 cell proliferation inhibition rate and PTEN, E-Cadherin, PTEN, GSK-3β proteins expression were significantly increased in miR-29a-mimics group(P<0.05), the cell clone formation rate, scratch healing rate, invasion number, and Ki-67, MMP-9, N-Cadherin, and Vimentin proteins expression were significantly reduced(P<0.05); compared with miR-29a-mimics group, the TG-905 cell proliferation inhibition rate and PTEN, E-Cadherin, PTEN, GSK-3β proteins expression were significantly reduced in miR-29a-mimics+Bpv group(P<0.05), the cell clone formation rate, scratch healing rate, invasion number, and Ki-67, MMP-9, N-Cadherin, and Vimentin proteins expression were significantly increased(P<0.05). Conclusion: Overexpression of miR-29a may up-regulate the expression of PTEN to inhibit the activation of PI3K/Akt/GSK-3β signaling pathway and inhibit the proliferation, invasion and migration of TG-905 cells.
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