Objective: To explore the effect of Circ-0032131 on the proliferation and secretion of inflammatory factors in osteoarthritis chondrocytes and its molecular mechanism. Methods: The cartilage tissue of 17 patients with osteoarthritis and normal tissues in our hospital were selected; chondrocytes CHON-001 were divided into control group, IL-1β group, IL-1β+si-Circ-0032131 group, IL-1β+si-NC group, IL-1β+miR-140-3p mimics group, IL-1β+miR-mimics-NC group, IL-1β+si-NC+miR-inhibitor-NC group, IL-1β+si-Circ-0032131+miR-inhibitor-NC group, IL-1β+si-Circ-0032131+miR-140-3p inhibitor group. Real-time fluorescent quantitative PCR(RT-qPCR) was used to detect the expression levels of Circ-0032131 and miR-140-3p; Western blot to detect protein expression; MTT to detect cell proliferation; plate experiment to detect cell clone formation; ELISA to detect TNF-α, IL-6 levels; dual luciferase reporter experiment verified the targeting relationship between Circ-0032131 and miR-140-3p. Results: Compared with normal tissues, the expression level of Circ-0032131 was increased and the expression level of miR-140-3p was decreased in cartilage tissue of patients with osteoarthritis(P<0.05). In chondrocytes induced by IL-1β, the expression levels of MMP1 and MMP13 were increased, the expression level of Collagen II was decreased, the OD value was decreased, the number of cell clone formation was decreased, the expression level of CyclinD1 was decreased, the expression level of P21 was increased, and the levels of TNF-α, IL-6 were increased(P<0.05). After Circ-0032131 silenced or miR-140-3p overexpressed, the OD value was increased, the number of cell clone formation was increased, the expression of CyclinD1 was increased, the expression of P21 was decreased, TNF-α, IL-6 levels were decreased in IL-1β-induced chondrocytes(P<0.05). Circ-0032131 targets miR-140-3p expression; interference with miR-140-3p could reverse the effect of silencing Circ-0032131 on the proliferation and secretion of inflammatory factors in osteoarthritis chondrocytes. Conclusions: Circ-0032131 may regulate IL-1β-induced chondrocyte proliferation and secretion of inflammatory factors by targeting miR-140-3p.
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