Objective:To explore the pathogen distributionandinflammatory factors in neonates withearly-onset sepsis(EOS) and late-onset sepsis(LOS).Methods:From October 2019 to December 2020, 60 cases of neonatal septicemia were selected, among them, 30 cases with EOS were regarded as early onset group, and 30 cases with LOS children as late-onset group. The levels of hypersensitive C-reactive protein(hs-CRP) and procalcitonin(PCT) were measured by turbidimetry and electrochemiluminescence; blood culture was used to detect pathogenic bacteria and Mic method was used for drug sensitivity test.Results:The incidence of gestational age, birth weight, birth asphyxia, maternal pregnancy infection, premature rupture of membranes and intrauterine distress in EOS children were significantly higher than those in LOS children(P<0.05). The number of strains infected with Gram-negative bacteria in the early-onset group was significantly higher than that in the late-onset group, while the number of strains infected with Gram-positive bacteria in the early-onset group was significantly lower than that in the late-onset group(P<0.05); the main pathogens in early onset group were Escherichia coli(26.67%) and Klebsiella pneumoniae(16.67%); the main pathogens in late onset group were coagulase negative staphylococcus(30.00%) and Staphylococcus aureus(16.67%); the resistance rates of gram negative bacteria and gram positive bacteria to penicillin and ampicillin were higher, while that to ceftriaxone was lower. Compared with those in the late-onset group, the levels of white blood cell count(WBC), hs-CRP and PCT in the early-onset group had no significant difference(P>0.05), while the level of platelet(PLT) was significantly lower(P<0.05). After 36 hours of treatment, the level of PLT in both groups was significantly higher, while PCT and hs-CRP were significantly lower(P<0.05), and there was no significant difference in WBC level(P>0.05).Conclusion:The main pathogenic bacteria composition, drug resistance and inflammatory cytokine levels of EOS and LOS are different, which has important reference value for early detection of disease, differentiation of disease types and rational use of antibiotics. |
