miR-338-3p调控头颈部鳞状细胞癌自噬、增殖、凋亡的作用及其机制-现代医学

miR-338-3p调控头颈部鳞状细胞癌自噬、增殖、凋亡的作用及其机制

单位：资阳市第一人民医院皮肤科, 四川资阳 641300

分类号：R739.91

出版年·卷·期（页码）：2022·50·第三期（273-279）

摘要：

目的：研究miR-338-3p调控头颈部鳞状细胞癌(HNSCC)自噬、增殖、凋亡的作用及其机制。方法：以实时荧光定量聚合酶链反应(qRT-PCR)检测miR-338-3p在HNSCC组织及癌旁组织中的表达。体外培养人头颈鳞癌细胞株PCI-37A，随机分为对照组、miR-338-3p mimics阴性对照组、miR-338-3p mimics组，分组转染后，以细胞计数试剂盒-8(CCK-8)实验和流式细胞术分别检测各组细胞增殖、凋亡情况；以吖啶橙染色检测各组细胞自噬水平；以qRT-PCR检测各组细胞miR-338-3p、RAS癌基因家族成员RAB23 mRNA水平；以免疫印迹法检测各组细胞RAB23、自噬和凋亡相关蛋白表达水平。结果：相比癌旁组织，miR-338-3p在HNSCC组织中表达明显下降(P<0.05)。相比对照组，miR-338-3p mimics组细胞相对生存率、自噬溶酶体相对含量、RAB23 mRNA和蛋白表达水平、B淋巴细胞瘤-2(Bcl-2)及Beclin-1、微管相关蛋白1轻链3B(LC3B)蛋白表达水平明显降低(P<0.05)，凋亡率、miR-338-3p水平、BCL2相关X蛋白(Bax)蛋白表达水平明显升高(P<0.05)；miR-338-3p mimics阴性对照组细胞各指标无明显变化(P>0.05)。结论：miR-338-3p在HNSCC组织中低表达；上调其表达，可抑制RAB23表达，降低癌细胞自噬水平，抑制癌细胞增殖并促使其凋亡。

Objective: To study the role and mechanism of miR-338-3p in regulating autophagy, proliferation and apoptosis of head and neck squamous cell carcinoma(HNSCC). Methods: The expression of miR-338-3p in HNSCC and adjacent tissues was detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR). Human head and neck squamous cell PCI-37A were cultured in vitro and randomly divided into control group, miR-338-3p mimics negative control group and miR-338-3p mimics group. After transfection, cell proliferation and apoptosis were detected by cell counting kit-8(CCK-8) assay and flow cytometry; the level of autophagy was detected by acridine orange staining; the levels of miR-338-3p and member RAS oncogene family RAB23 mRNA were detected by qRT-PCR; the expression levels of RAB23, autophagy and apoptosis related proteins were detected by Western blot. Results: Compared with that in the adjacent tissues, the expression of miR-338-3p in HNSCC was significantly lower(P<0.05). Compared with those in the control group, the relative survival rate, the relative content of autophagy lysosome, the expression levels of RAB23 mRNA and protein, the expression levels of B-cell lymphoma-2(Bcl-2), Beclin-1 and microtubule-associated protein 1 light chain 3B(LC3B) were significantly lower in miR-338-3p mimics group(P<0.05), the apoptosis rate, level of miR-338-3p and expression level of BCL2-associated X protein(Bax) protein were significantly higher(P<0.05); in miR-338-3p mimics negative control group, there were no significant changes in cell indexes(P>0.05). Conclusion: MiR-338-3p is down-regulated in HNSCC. Up-regulation of miR-338-3p can inhibit RAB23 expression, reduce autophagy level, inhibit proliferation and promote apoptosis of HNSCC.

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