Objective: To investigate the effect of circ_0003204 on renal tubular epithelial cell injury induced by high glucose(HG) and its mechanism. Methods: The renal tubular epithelial cells HK-2 were cultured in vitro and treated with 30 mmol·L-1 HG for 24 h, and then the expressions of circ_0003204 and miR-346 in the cells were detected by qRT-PCR. The dual luciferase reporter gene experiment verified the regulatory relationship between circ_0003204 and miR-346. After HK-2 cells transfected with circ_0003204 small interfering RNA or miR-346 mimic, or co-transfected with circ_0003204 small interfering RNA and miR-346 inhibitor, respectively, they were treated with 30 mmol·L-1 HG for 24 h. And then CCK-8 method was used to detect the inhibition rate of cell proliferation. Flow cytometry was used to detect cell apoptosis. The kits were used to detect the content of lactate dehydrogenase(LDH), interleukin(IL)-6 and IL-10 in cell culture supernatant and the activity of reactive oxygen(ROS), superoxide dismutase(SOD) and the content of malonaldehyde(MDA) in the cells. Results: After HK-2 cells treated with HG, the expression of circ_0003204 in the cells was increased(P<0.05), but the expression of miR-346 was decreased(P<0.05). circ_0003204 negatively regulated the expression of miR-346 in HK-2 cells. After the HK-2 cells that interfered with circ_0003204 or overexpressed miR-346 were treated with HG, the cell proliferation inhibition rate and apoptosis rate were reduced(P<0.05), the content of LDH and IL-6 in the culture supernatant, and the activity of ROS and the content of MDA in the cells were reduced(P<0.05), but the content of IL-10 in the culture supernatant and the activity of SOD in cells were increased(P<0.05). Interfering with miR-346 reversed the effect of interfering with circ_0003204 on HG-induced HK-2 cell apoptosis, oxidative stress and inflammatory factor expression. Conclusion: Interfering with circ_0003204 may inhibit HG-induced HK-2 cell apoptosis, oxidative stress and inflammation by targeting up-regulation of miR-346. |
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