Objective: To study the early changes of serum miR-195-5p and interleukin-17(IL-17) levels in children with Mycoplasma pneumonia(MPP) and their diagnostic value. Methods: A total of 150 children with MPP who visited our hospital's Pediatric Respiratory Clinic from February 2018 to February 2019 were selected as MPP group, and 50 children with healthy physical examination were selected as control group during the same period. Real-time fluorescent quantitative PCR(qRT-PCR) method was used to determine the expression of miR-195-5p in the serum of children in each group. Enzyme-linked immunosorbent assay(ELISA) was used to detect the level of serum IL-17 of children in the two groups. The levels of serum miR-195-5p and IL-17 in each group were compared. Pearson method was used to analyze the correlation between serum miR-195-5p and IL-17 levels. Receiver operating characteristic (ROC) curve was drawn to evaluate serum miR-195-5p and IL-17 levels and the early predictive value of the combined detection of the two for MPP. Logistic regression was used to analyze the influencing factors of MPP. Results: Compared with the control group, the serum miR-195-5p level of children in the MPP group was significantly decreased, and the serum IL-17 level was significantly increased in the MPP group(both P<0.05). Compared with before the treatment, the level of serum miR-195-5p in children with MPP increased significantly and the level of serum IL-17 decreased significantly after treatment(both P<0.05). Pearson analysis showed that serum miR-195-5p and IL-17 levels in children with MPP were negatively correlated(P<0.05). ROC curve analysis results showed that the area under the curve(AUC) of serum miR-195-5p for diagnosis of MPP was 0.875, with the sensitivity being 82.0%, the specificity being 88.2%, and the cut-off value being 3.869. The AUC of IL-17 for diagnosis of MPP was 0.736, with the sensitivity being 66.0%, the specificity being 74.1%, and the cut-off value being 152.919 ng·ml-1. The AUC of the combined diagnosis of MPP was 0.903, with the sensitivity being 96.0%, and the specificity being 97.1%. Multivariate Logistic regression analysis showed that serum miR-195-5p and IL-17 were the influencing factors of MPP. Conclusion: Serum miR-195-5p expression is low in children with MPP, and IL-17 expression is high. The combined detection of the two can significantly improve the diagnostic accuracy of MPP, which is more conducive to clinical medication guidance. |
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