Objective: To explore the efficacy and safety of treatment with expanded ex vivo autologous peripheral blood mononuclear cells(PBMC) in patients with amyotrophic lateral sclerosis(ALS). Methods: A total of 28 ALS patients were selected and admitted to the Department of Neurology, Nanjing Second Hospital from September 2019 to August 2020. PBMC was isolated from peripheral blood of the subjects and then cultured and amplified for 42 days after stimulated with phytohemagglutinin-L 1.25 μg·ml-1 and IL-2 5×103·IU·ml-1 in vitro for 3 days. According to the standard of blood transfusion, 5×107 PBMC cells were infused intravenously into patients for one round of treatment, and the above-mentioned PBMC culture and amplification were taken for 6 consecutive rounds of treatment as a course of treatment. The trial had total six cycles. The Rasch-Built Overall ALS Disability Scale(ROADS) and the Revised ALS Functional Rating Scale(ALSFRS-R) were used to determine outcomes. Results: There were also significant improvements when we compared the score of ROADS after six cycles treatment with that of before the treatment(baseline)(P<0.05). However, there was no difference in the score of ALSFRS-R after 3 months treatment than that of baseline(P>0.05) although the score decreased after the treatment. In 28 patients, 16 patients felt improvement(57.1%). The improved symptoms included muscle tone, muscle strength, speech loss, swallowing difficulties, smooth breathing, etc. No serious adverse reactions were found in the treatment. Conclusion: The autologous PBMC cell therapy is safe and effective for ALS patients. Good respiratory support,closely monitored for vital signs and adverse reactions are the key to ensure the success of autotransfusion of PBMC in patients with ALS. |
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