妊娠期高血压疾病患者外周血miR-206和miR-483水平变化与妊娠结局的关系研究-现代医学

妊娠期高血压疾病患者外周血miR-206和miR-483水平变化与妊娠结局的关系研究

单位：河北中石油中心医院产科, 河北廊坊 065000

分类号：R714.2

出版年·卷·期（页码）：2023·51·第七期（923-928）

摘要：

目的: 探究妊娠期高血压疾病患者外周血微小RNA(miR)-206和miR-483水平变化与妊娠结局的关系。方法: 选取2021年1月至2022年6月在本院接受治疗的180例妊娠期高血压疾病患者,根据妊娠结局,将患者分为良好妊娠结局组120例和不良妊娠结局组60例。采用实时荧光定量PCR(qRT-PCR)法对患者血清中miR-206、miR-483表达水平进行检测。Spearman法分析妊娠期高血压疾病患者血清中miR-206和miR-483水平与妊娠结局的关系;对影响妊娠期高血压疾病患者妊娠结局的因素进行Logistic回归分析;受试者工作特征(ROC)曲线分析血清miR-206和miR-483水平对妊娠期高血压疾病患者妊娠结局的预测价值。结果: 与良好妊娠结局组比较,不良妊娠结局组患者血清miR-206水平显著升高(P<0.05),miR-483水平显著降低(P<0.05);妊娠期高血压疾病患者血清miR-206水平与妊娠结局呈正相关(r=0.605,P<0.001),miR-483水平与妊娠结局呈负相关(r=-0.663,P<0.001);Logistic回归分析发现,疾病严重程度、miR-206是影响妊娠期高血压疾病患者妊娠结局的危险因素(P<0.05),miR-483是影响患者妊娠结局的保护因素(P<0.05);血清miR-206、miR-483联合预测妊娠期高血压疾病患者妊娠结局的ROC曲线下面积(AUC)为0.961,均优于其各自单独预测(均P<0.05)。结论: 娠期高血压疾病患者外周血miR-206水平升高,miR-483水平降低,与妊娠结局有关,能够对患者妊娠结局进行评估。

Objective: To explore the relationship between the changes of microRNA(miR)-206 and miR-483 levels in peripheral blood of patients with hypertensive disorder complicating pregnancy and pregnancy outcome. Methods: A total of 180 patients with hypertensive disorder complicating pregnancy who were treated in our hospital from January 2021 to June 2022 were selected,according to the pregnancy outcome, the patients were divided into good pregnancy outcome group(120 cases) and poor pregnancy outcome group(60 cases). The expression levels of miR-206 and miR-483 in patients' serum were detected by real-time fluorescence quantitative PCR(qRT-PCR). Spearman method was applied to analyze the relationship between the levels of miR-206 and miR-483 in serum of patients with hypertensive disorder complicating pregnancy and pregnancy outcome; Logistic regression analysis was applied to analyze the factors affecting the pregnancy outcome of patients with hypertensive disorder complicating pregnancy; and the predictive value of serum miR-206 and miR-483 levels on pregnancy outcome in patients with hypertensive disorder complicating pregnancy was analyzed by ROC curve. Results: Compared with the good pregnancy outcome group, the serum miR-206 level in the poor pregnancy outcome group was significantly higher(P<0.05) and the miR-483 level was significantly lower(P<0.05); the serum miR-206 level in patients with hypertensive disorder complicating pregnancy was positively correlated with pregnancy outcome(r=0.605, P<0.001), while the miR-483 level was negatively correlated with pregnancy outcome(r=-0.663, P<0.001); Logistic regression analysis showed that disease severity and miR-206 were risk factors affecting pregnancy outcome of patients with hypertensive disorder complicating pregnancy(P<0.05), and miR-483 was protective factor affecting pregnancy outcome(P<0.05); the area under the ROC curve(AUC) of serum miR-206 combined with miR-483 predicts the pregnancy outcome of patients with hypertensive disorder complicating pregnancy was 0.961, which was better than singular prediction(all P<0.05). Conclusion: The level of miR-206 in peripheral blood of hypertensive patients during pregnancy is increased, miR-483 level is decreased, which are related to the pregnancy outcome, and can be used to evaluate the pregnancy outcome of patients.

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