Objective: To understand the epidemiological characteristics of HIV/AIDS(HIV/AIDS) in Pukou District, Nanjing, and to provide a basis for formulating prevention and control strategies. Methods: Based on the HIV/AIDS epidemic report data reported in Pukou District, Nanjing, for 2018-2022 from the AIDS monitoring system of the Chinese Center for Disease Control and Prevention, we sorted out and analyzed the epidemic situation, three distributions, infection routes, sample sources and late detection. Results: From 2018 to 2022, a total of 123 AIDS cases were reported in Pukou District, Nanjing, and the number of newly reported cases increased year by year(APC=21.70, P<0.05). The cases reportedwere mainly male, with a sex ratio of 6.69:1, their age was mainly ≥ 60 years old(32.52%), their occupations were mainly farmers, houseworker or unemployed(23.57%), their education level was mainly junior college or above(38.21%), however the reported cases with primary school and below was on the rise. The number of cases reported in all the five streets of the region was reported. The infection route was mainly sexual transmission, detection consultation was mainly the discovery route(43.09%), and the late detection rate was 27.06%. Conclusion: The AIDS epidemic in Pukou District is on the rise year by year. The cases are mainly men, the elderly and farmers. Comprehensive intervention measures should be taken to intervene in the current AIDS epidemic.