RHOJ在胃癌中的表达及其对预后的影响-现代医学

RHOJ在胃癌中的表达及其对预后的影响

单位：1. 苏州大学附属第一医院肿瘤科, 江苏苏州 215006;
2. 苏州大学附属第一医院中心ICU, 江苏苏州 215006

分类号：R735.2

出版年·卷·期（页码）：2024·52·第九期（1321-1329）

摘要：

目的： 基于数据库分析RHOJ在胃癌患者中的表达及其与胃癌患者临床病理特征、预后、肿瘤微环境免疫浸润的关系，并探讨相关机制。方法： 通过检索UALCAN和HPA数据库，了解RHOJ在胃癌组织及正常组织中的差异表达情况，并了解RHOJ的亚细胞表达；通过检索TCGA数据库、GEO数据库GSE84426、GSE84437数据集分别下载了415例、76例、434例胃癌组织RNA-Seq数据和临床基线资料，分别分析TCGA数据库和GEO数据库中的RHOJ表达量与临床病理特征的关系，Cox分析TCGA数据库RHOJ表达量和临床基线资料与患者预后的关系；利用KM-plotter数据库分析RHOJ表达与胃癌患者预后的关系并绘制生存曲线及免疫治疗相关的受试者工作特征曲线(ROC曲线)；通过检索TISIDB数据库评估RHOJ的表达与胃癌肿瘤微环境免疫浸润的关系；通过STRING数据库分析与RHOJ相关作用的蛋白，并进行基因本体功能注释及KEGG通路富集分析。结果： 与正常对照相比，RHOJ在胃癌组织中呈低表达，与胃癌肿瘤免疫浸润有关，有助于评估胃癌免疫治疗疗效，RHOJ在胃癌患者中低表达是提示患者预后良好的指标。结论： RHOJ可作为影响胃癌患者预后的一种生物学标志物，并且具有良好的诊断价值。

Objective: To analyze the expression of RHOJ in patients with gastric cancer and its relationship with clinicopathological features, prognosis and immune infiltration of tumor microenvironment based on database, and to explore the related mechanism. Methods: By searching UALCAN and HPA databases, we investigated the differential expression of RHOJ in gastric cancer and normal tissues, and the subcellular expression of RHOJ. The TCGA database and GEO database were searched. RNA-Seq data and clinical baseline data of 415, 76 and 434 cases of gastric cancer tissues were downloaded from Omnibus database GSE84426 and GSE84437, respectively. The relationship between RHOJ expression and clinicopathological features in TCGA and GEO databases was analyzed. The correlation between RHOJ expression in TCGA database and clinical baseline data and prognosis was analyzed by Cox regression analysis. KM-plotter database was used to analyze the relationship between RHOJ expression and prognosis of gastric cancer patients, and the survival curve and immune treatment-related receiver operating characteristic(ROC) curve were drawn. TISIDB database was searched to evaluate the relationship between RHOJ expression and immune invasion of gastric cancer microenvironment. Proteins associated with RHOJ were analyzed through STRING database. Gene ontology(GO) function annotation and KEGG pathway enrichment analyses were also conducted. Results: Compared with the normal control group, RHOJ had a low expression in gastric cancer tissues, which was related to immune invasion of gastric cancer tumors and was helpful to evaluate the efficacy of immunotherapy for gastric cancer. The low expression of RHOJ in patients with gastric cancer was an indicator of good prognosis. Conclusion: RHOJ can be used as a biological marker affecting the prognosis of patients with gastric cancer, and has a good diagnostic value.

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